MicroscopyMatching: Towards a Ready-to-use Framework for Microscopy Image Analysis in Diverse Conditions

Analyzing microscopy images to extract biological object properties (e.g., their morphological organization, temporal dynamics, and population density) is fundamental to various biomedical research. Yet conducting this manually is costly and time-consuming. Though deep learning-based approaches have been explored to automate this process, the substantial diversity of microscopy analysis settings in practice (including variations of biological object types, sample processing protocols, imaging equipment, and analysis tasks, etc.) often renders them ineffective. As a result, these approaches typically require extensive adaptation for different settings, which, however, can impose burdens that are often practically unsustainable for laboratories, forcing biomedical researchers to still commonly rely on manual analysis, thereby severely bottlenecking the pace of biomedical research progress. This situation has created a pressing and long-standing need for a reliable and broadly applicable microscopy image analysis tool, yet such a tool is still missing. To address this gap, we present the first ready-to-use microscopy image analysis framework, MicroscopyMatching, that can reliably perform key analysis tasks (including segmentation, tracking, and counting) across diverse microscopy analysis settings. From a fundamentally different perspective, MicroscopyMatching reformulates diverse microscopy image analysis tasks as a unified matching problem, effectively handling this problem by exploiting the robust matching capability from pre-trained latent diffusion models.

Beyond Individual Intelligence: Surveying Collaboration, Failure Attribution, and Self-Evolution in LLM-based Multi-Agent Systems

LLM-based autonomous agents have demonstrated strong capabilities in reasoning, planning, and tool use, yet remain limited when tasks require sustained coordination across roles, tools, and environments. Multi-agent systems address this through structured collaboration among specialized agents, but tighter coordination also amplifies a less explored risk: errors can propagate across agents and interaction rounds, producing failures that are difficult to diagnose and rarely translate into structural self-improvement. Existing surveys cover individual agent capabilities, multi-agent collaboration, or agent self-evolution separately, leaving the causal dependencies among them unexamined. This survey provides a unified review organized around four causally linked stages, which we term the LIFE progression: Lay the capability foundation, Integrate agents through collaboration, Find faults through attribution, and Evolve through autonomous self-improvement. For each stage, we provide systematic taxonomies and formally characterize the dependencies between adjacent stages, revealing how each stage both depends on and constrains the next. Beyond synthesizing existing work, we identify open challenges at stage boundaries and propose a cross-stage research agenda for closed-loop multi-agent systems capable of continuously diagnosing failures, reorganizing structures, and refining agent behaviors, extending current coordination frameworks toward more self-organizing forms of collective intelligence. By bridging these previously fragmented research threads, this survey aims to offer both a systematic reference and a conceptual roadmap toward autonomous, self-improving multi-agent intelligence.

When Policy Entropy Constraint Fails: Preserving Diversity in Flow-based RLHF via Perceptual Entropy

RLHF is widely used to align flow-matching text-to-image models with human preferences, but often leads to severe diversity collapse after fine-tuning. In RL, diversity is often assumed to correlate with policy entropy, motivating entropy regularization. However, we show this intuition breaks in flow models: policy entropy remains constant, even while perceptual diversity collapses. We explain this mismatch both theoretically and empirically: the constant entropy arises from the fixed, pre-defined noise schedule, while the diversity collapse is driven by the mode-seeking nature of policy gradients. As a result, policy entropy fails to prevent the model from converging to a narrow high-reward region in the perceptual space. To this end, we introduce perceptual entropy that captures diversity in a perceptual space and maintains the property of standard entropy. Building upon this insight, we propose two entropy-regularized strategies, Perceptual Entropy Constraint and Perceptual Constraints on Generation Space, to preserve perceptual diversity and improve the quality. Experiments across two base models, neural and rule-based rewards, and three perceptual spaces demonstrate consistent gains in the quality-diversity trade-off; PEC achieves the best overall score of 0.734 (vs. baseline's 0.366); a complementary setting of PEC further reaches a diversity average of 0.989 (vs. baseline's 0.047). Our project page (https://xiaofeng-tan.github.io/projects/PEC) is publicly available.

RAWild: Sensor-Agnostic RAW Object Detection via Physics-Guided Curve and Grid Modeling

Camera sensor RAW data offers intrinsic advantages for object detection, including deeper bit depth, preserved physical information, and freedom from image signal processor (ISP) distortions. However, varying exposure conditions, spectral sensitivities, and bit depths across devices introduce substantially larger domain gaps than sRGB, making sensor-agnostic generalization a fundamental challenge. In this study, we present \textbf{RAWild}, a physics-guided global-local tone mapping framework for sensor-agnostic RAW object detection. By factoring sensor-induced variations into a global tonal correction and a spatially adaptive local color adjustment, both driven by RAW distribution priors, our framework enables a single network to train jointly across heterogeneous sensors. To further support cross-sensor generalization, we construct a physics-based RAW simulation pipeline that synthesizes realistic sensor outputs spanning diverse spectral sensitivities, illuminants, and sensor non-idealities. Extensive experiments across multiple RAW benchmarks covering bit depths from 10 to 24 demonstrate state-of-the-art (SOTA) performance under single-dataset, mixed-dataset, and challenging robustness settings.

Knowledge-Graph Paths as Intermediate Supervision for Self-Evolving Search Agents

Self-evolving search agents reduce reliance on human-written training questions by generating and solving their own search tasks. We build on Search Self-Play (SSP), a representative Proposer and Solver framework in which questions are generated and answered via multi-step search and reasoning. In practice, however, SSP faces two bottlenecks: the Proposer constructs questions from isolated answer entities without relational context, yielding many invalid or unverifiable questions in early self-play training, while the Solver receives only a binary outcome reward that discards useful signal from partially on-track search trajectories. We address both bottlenecks by reusing knowledge-graph paths as construction-derived intermediate supervision for both question construction and reward shaping. First, we ground question construction in LLM-guided knowledge-graph subgraphs, providing relational context for the Proposer. Second, we observe that constructing and solving a multi-hop question can involve overlapping intermediate entities: the factual bridges used to formulate the question may provide approximate waypoints for answering it. Exploiting this overlap, we introduce Waypoint Coverage Reward (WCR), which grants graded partial credit to incorrect Solver trajectories according to their coverage of entities on the construction path, while preserving full reward for correct answers. Across seven QA benchmarks and nine model configurations, our approach improves the average score over standard SSP in all configurations, including notable gains on multi-hop QA tasks. These results suggest that knowledge-graph paths can be reused as lightweight intermediate supervision, providing both relational guidance and process feedback without additional task-specific human annotations or manually labeled process steps.

FluxFlow: Conservative Flow-Matching for Astronomical Image Super-Resolution

Ground-to-space astronomical super-resolution requires recovering space-quality images from ground-based observations that are simultaneously limited by pixel sampling resolution and atmospheric seeing, which imposes a stochastic, spatially varying PSF that cannot be resolved through upsampling alone. Existing methods rely on synthetic training pairs that fail to capture real atmospheric statistics and are prone to either over-smoothed reconstructions or hallucination sources with no physical counterpart in the observed sky. We propose FluxFlow, a conservative pixel-space flow-matching framework that incorporates observation uncertainty and source-region importance weights during training, and a training-free Wiener-regularized test-time correction to suppress hallucination sources while preserving recovered detail. We further construct the DESI--HST Dataset, the large-scale real-world benchmark comprising 19,500 real co-registered ground-to-space image pairs with real atmospheric PSF variation. Experiments demonstrate that FluxFlow consistently outperforms existing baseline methods in both photometric and scientific accuracy.

Generalizable CT-Free PET Attenuation and Scatter Correction for Pediatric Patients

Computed tomography (CT)-based attenuation and scatter correction improves quantitative PET but adds radiation exposure that is particularly undesirable in pediatric imaging. Existing CT-free methods are commonly trained in homogeneous settings and often degrade under scanner or radiotracer shifts, which limits their clinical utility. We propose the Generalizable PET Correction Network (GPCN), a dual-domain network for domain-robust CT-free PET attenuation and scatter correction. GPCN combines a multi-band contextual refinement module, which models pediatric anatomical variability through wavelet-based multiscale decomposition and long-range spatial context modeling, with a frequency-aware spectral decoupling module, which performs coordinate-conditioned amplitude/phase refinement in the Fourier domain. By synergizing multi-band spatial contextual modeling with asymmetric frequency-spectrum decoupling, the network explicitly separates invariant topological structures from domain-specific noise, thereby achieving precise quantitative recovery of both anatomical organs and focal lesions. This design aims to separate anatomy-dominant structures from domain-sensitive spectral residuals and to improve robustness across heterogeneous imaging conditions. We train and evaluate the method on 1085 pediatric whole-body PET scans acquired with two scanners and five radiotracers. In both joint training and zero-shot cross-domain evaluation, GPCN outperforms representative baselines and maintains stable quantitative accuracy on unseen scanner-tracer combinations. The method is further supported by ablation, region-wise quantitative analysis, and downstream segmentation experiments. In our cohort, the CT component of the conventional protocol corresponded to an average effective dose of 10.8 mSv, indicating the potential clinical value of reliable CT-free correction for pediatric PET.

OptiVerse: A Comprehensive Benchmark towards Optimization Problem Solving

While Large Language Models (LLMs) demonstrate remarkable reasoning, complex optimization tasks remain challenging, requiring domain knowledge and robust implementation. However, existing benchmarks focus narrowly on Mathematical Programming and Combinatorial Optimization, hindering comprehensive evaluation. To address this, we introduce OptiVerse, a comprehensive benchmark of 1,000 curated problems spanning neglected domains, including Stochastic Optimization, Dynamic Optimization, Game Optimization, and Optimal Control, across three difficulty levels: Easy, Medium, and Hard. The experiments with 22 LLMs of different sizes reveal sharp performance degradation on hard problems, where even advanced models like GPT-5.2 and Gemini-3 struggle to exceed 27% accuracy. Through error analysis, we identify that modeling & logic errors remain the primary bottleneck. Consequently, we propose a Dual-View Auditor Agent that improves the accuracy of the LLM modeling process without introducing significant time overhead. OptiVerse will serve as a foundational platform for advancing LLMs in solving complex optimization challenges.

Dual-Cluster Memory Agent: Resolving Multi-Paradigm Ambiguity in Optimization Problem Solving

Large Language Models (LLMs) often struggle with structural ambiguity in optimization problems, where a single problem admits multiple related but conflicting modeling paradigms, hindering effective solution generation. To address this, we propose Dual-Cluster Memory Agent (DCM-Agent) to enhance performance by leveraging historical solutions in a training-free manner. Central to this is Dual-Cluster Memory Construction. This agent assigns historical solutions to modeling and coding clusters, then distills each cluster's content into three structured types: Approach, Checklist, and Pitfall. This process derives generalizable guidance knowledge. Furthermore, this agent introduces Memory-augmented Inference to dynamically navigate solution paths, detect and repair errors, and adaptively switch reasoning paths with structured knowledge. The experiments across seven optimization benchmarks demonstrate that DCM-Agent achieves an average performance improvement of 11%- 21%. Notably, our analysis reveals a ``knowledge inheritance'' phenomenon: memory constructed by larger models can guide smaller models toward superior performance, highlighting the framework's scalability and efficiency.

Towards Fine-grained Temporal Perception: Post-Training Large Audio-Language Models with Audio-Side Time Prompt

Large Audio-Language Models (LALMs) enable general audio understanding and demonstrate remarkable performance across various audio tasks. However, these models still face challenges in temporal perception (e.g., inferring event onset and offset), leading to limited utility in fine-grained scenarios. To address this issue, we propose Audio-Side Time Prompt and leverage Reinforcement Learning (RL) to develop the TimePro-RL framework for fine-grained temporal perception. Specifically, we encode timestamps as embeddings and interleave them within the audio feature sequence as temporal coordinates to prompt the model. Furthermore, we introduce RL following Supervised Fine-Tuning (SFT) to directly optimize temporal alignment performance. Experiments demonstrate that TimePro-RL achieves significant performance gains across a range of audio temporal tasks, such as audio grounding, sound event detection, and dense audio captioning, validating its robust effectiveness.