A systematic evaluation of methods for cell phenotype classification using single-cell RNA sequencing data

Background: Single-cell RNA sequencing (scRNA-seq) yields valuable insights about gene expression and gives critical information about complex tissue cellular composition. In the analysis of single-cell RNA sequencing, the annotations of cell subtypes are often done manually, which is time-consuming and irreproducible. Garnett is a cell-type annotation software based the on elastic net method. Besides cell-type annotation, supervised machine learning methods can also be applied to predict other cell phenotypes from genomic data. Despite the popularity of such applications, there is no existing study to systematically investigate the performance of those supervised algorithms in various sizes of scRNA-seq data sets. Methods and Results: This study evaluates 13 popular supervised machine learning algorithms to classify cell phenotypes, using published real and simulated data sets with diverse cell sizes. The benchmark contained two parts. In the first part, we used real data sets to assess the popular supervised algorithms' computing speed and cell phenotype classification performance. The classification performances were evaluated using AUC statistics, F1-score, precision, recall, and false-positive rate. In the second part, we evaluated gene selection performance using published simulated data sets with a known list of real genes. Conclusion: The study outcomes showed that ElasticNet with interactions performed best in small and medium data sets. NB was another appropriate method for medium data sets. In large data sets, XGB works excellent. Ensemble algorithms were not significantly superior to individual machine learning methods. Adding interactions to ElasticNet can help, and the improvement was significant in small data sets.

UAV-Enabled Wireless Power Transfer: A Tutorial Overview

Unmanned aerial vehicle (UAV)-enabled wireless power transfer (WPT) has recently emerged as a promising technique to provide sustainable energy supply for widely distributed low-power ground devices (GDs) in large-scale wireless networks. Compared with the energy transmitters (ETs) in conventional WPT systems which are deployed at fixed locations, UAV-mounted aerial ETs can fly flexibly in the three-dimensional (3D) space to charge nearby GDs more efficiently. This paper provides a tutorial overview on UAV-enabled WPT and its appealing applications, in particular focusing on how to exploit UAVs' controllable mobility via their 3D trajectory design to maximize the amounts of energy transferred to all GDs in a wireless network with fairness. First, we consider the single-UAV-enabled WPT scenario with one UAV wirelessly charging multiple GDs at known locations. To solve the energy maximization problem in this case, we present a general trajectory design framework consisting of three innovative approaches to optimize the UAV trajectory, which are multi-location hovering, successive-hover-and-fly, and time-quantization-based optimization, respectively. Next, we consider the multi-UAV-enabled WPT scenario where multiple UAVs cooperatively charge many GDs in a large area. Building upon the single-UAV trajectory design, we propose two efficient schemes to jointly optimize multiple UAVs' trajectories, based on the principles of UAV swarming and GD clustering, respectively. Furthermore, we consider two important extensions of UAV-enabled WPT, namely UAV-enabled wireless powered communication networks (WPCN) and UAV-enabled wireless powered mobile edge computing (MEC).