Simulation of Nanorobots with Artificial Intelligence and Reinforcement Learning for Advanced Cancer Cell Detection and Tracking

Nanorobots are a promising development in targeted drug delivery and the treatment of neurological disorders, with potential for crossing the blood-brain barrier (BBB). These small devices leverage advancements in nanotechnology and bioengineering for precise navigation and targeted payload delivery, particularly for conditions like brain tumors, Alzheimer's disease, and Parkinson's disease. Recent progress in artificial intelligence (AI) and machine learning (ML) has improved the navigation and effectiveness of nanorobots, allowing them to detect and interact with cancer cells through biomarker analysis. This study presents a new reinforcement learning (RL) framework for optimizing nanorobot navigation in complex biological environments, focusing on cancer cell detection by analyzing the concentration gradients of surrounding biomarkers. We utilize a computer simulation model to explore the behavior of nanorobots in a three-dimensional space with cancer cells and biological barriers. The proposed method uses Q-learning to refine movement strategies based on real-time biomarker concentration data, enabling nanorobots to autonomously navigate to cancerous tissues for targeted drug delivery. This research lays the groundwork for future laboratory experiments and clinical applications, with implications for personalized medicine and less invasive cancer treatments. The integration of intelligent nanorobots could revolutionize therapeutic strategies, reducing side effects and enhancing treatment effectiveness for cancer patients. Further research will investigate the practical deployment of these technologies in medical settings, aiming to unlock the full potential of nanorobotics in healthcare.

Demographic Predictability in 3D CT Foundation Embeddings

Self-supervised foundation models have recently been successfully extended to encode three-dimensional (3D) computed tomography (CT) images, with excellent performance across several downstream tasks, such as intracranial hemorrhage detection and lung cancer risk forecasting. However, as self-supervised models learn from complex data distributions, questions arise concerning whether these embeddings capture demographic information, such as age, sex, or race. Using the National Lung Screening Trial (NLST) dataset, which contains 3D CT images and demographic data, we evaluated a range of classifiers: softmax regression, linear regression, linear support vector machine, random forest, and decision tree, to predict sex, race, and age of the patients in the images. Our results indicate that the embeddings effectively encoded age and sex information, with a linear regression model achieving a root mean square error (RMSE) of 3.8 years for age prediction and a softmax regression model attaining an AUC of 0.998 for sex classification. Race prediction was less effective, with an AUC of 0.878. These findings suggest a detailed exploration into the information encoded in self-supervised learning frameworks is needed to help ensure fair, responsible, and patient privacy-protected healthcare AI.

Revisiting Lesion Tracking in 3D Total Body Photography

Melanoma is the most deadly form of skin cancer. Tracking the evolution of nevi and detecting new lesions across the body is essential for the early detection of melanoma. Despite prior work on longitudinal tracking of skin lesions in 3D total body photography, there are still several challenges, including 1) low accuracy for finding correct lesion pairs across scans, 2) sensitivity to noisy lesion detection, and 3) lack of large-scale datasets with numerous annotated lesion pairs. We propose a framework that takes in a pair of 3D textured meshes, matches lesions in the context of total body photography, and identifies unmatchable lesions. We start by computing correspondence maps bringing the source and target meshes to a template mesh. Using these maps to define source/target signals over the template domain, we construct a flow field aligning the mapped signals. The initial correspondence maps are then refined by advecting forward/backward along the vector field. Finally, lesion assignment is performed using the refined correspondence maps. We propose the first large-scale dataset for skin lesion tracking with 25K lesion pairs across 198 subjects. The proposed method achieves a success rate of 89.9% (at 10 mm criterion) for all pairs of annotated lesions and a matching accuracy of 98.2% for subjects with more than 200 lesions.

SegCol Challenge: Semantic Segmentation for Tools and Fold Edges in Colonoscopy data

Colorectal cancer (CRC) remains a leading cause of cancer-related deaths worldwide, with polyp removal being an effective early screening method. However, navigating the colon for thorough polyp detection poses significant challenges. To advance camera navigation in colonoscopy, we propose the Semantic Segmentation for Tools and Fold Edges in Colonoscopy (SegCol) Challenge. This challenge introduces a dataset from the EndoMapper repository, featuring manually annotated, pixel-level semantic labels for colon folds and endoscopic tools across selected frames from 96 colonoscopy videos. By providing fold edges as anatomical landmarks and depth discontinuity information from both fold and tool labels, the dataset is aimed to improve depth perception and localization methods. Hosted as part of the Endovis Challenge at MICCAI 2024, SegCol aims to drive innovation in colonoscopy navigation systems. Details are available at https://www.synapse.org/Synapse:syn54124209/wiki/626563, and code resources at https://github.com/surgical-vision/segcol_challenge .

Unified Local and Global Attention Interaction Modeling for Vision Transformers

We present a novel method that extends the self-attention mechanism of a vision transformer (ViT) for more accurate object detection across diverse datasets. ViTs show strong capability for image understanding tasks such as object detection, segmentation, and classification. This is due in part to their ability to leverage global information from interactions among visual tokens. However, the self-attention mechanism in ViTs are limited because they do not allow visual tokens to exchange local or global information with neighboring features before computing global attention. This is problematic because tokens are treated in isolation when attending (matching) to other tokens, and valuable spatial relationships are overlooked. This isolation is further compounded by dot-product similarity operations that make tokens from different semantic classes appear visually similar. To address these limitations, we introduce two modifications to the traditional self-attention framework; a novel aggressive convolution pooling strategy for local feature mixing, and a new conceptual attention transformation to facilitate interaction and feature exchange between semantic concepts. Experimental results demonstrate that local and global information exchange among visual features before self-attention significantly improves performance on challenging object detection tasks and generalizes across multiple benchmark datasets and challenging medical datasets. We publish source code and a novel dataset of cancerous tumors (chimeric cell clusters).

Interpretable Droplet Digital PCR Assay for Trustworthy Molecular Diagnostics

Accurate molecular quantification is essential for advancing research and diagnostics in fields such as infectious diseases, cancer biology, and genetic disorders. Droplet digital PCR (ddPCR) has emerged as a gold standard for achieving absolute quantification. While computational ddPCR technologies have advanced significantly, achieving automatic interpretation and consistent adaptability across diverse operational environments remains a challenge. To address these limitations, we introduce the intelligent interpretable droplet digital PCR (I2ddPCR) assay, a comprehensive framework integrating front-end predictive models (for droplet segmentation and classification) with GPT-4o multimodal large language model (MLLM, for context-aware explanations and recommendations) to automate and enhance ddPCR image analysis. This approach surpasses the state-of-the-art models, affording 99.05% accuracy in processing complex ddPCR images containing over 300 droplets per image with varying signal-to-noise ratios (SNRs). By combining specialized neural networks and large language models, the I2ddPCR assay offers a robust and adaptable solution for absolute molecular quantification, achieving a sensitivity capable of detecting low-abundance targets as low as 90.32 copies/{\mu}L. Furthermore, it improves model's transparency through detailed explanation and troubleshooting guidance, empowering users to make informed decisions. This innovative framework has the potential to benefit molecular diagnostics, disease research, and clinical applications, especially in resource-constrained settings.

UNet++ and LSTM combined approach for Breast Ultrasound Image Segmentation

Breast cancer stands as a prevalent cause of fatality among females on a global scale, with prompt detection playing a pivotal role in diminishing mortality rates. The utilization of ultrasound scans in the BUSI dataset for medical imagery pertaining to breast cancer has exhibited commendable segmentation outcomes through the application of UNet and UNet++ networks. Nevertheless, a notable drawback of these models resides in their inattention towards the temporal aspects embedded within the images. This research endeavors to enrich the UNet++ architecture by integrating LSTM layers and self-attention mechanisms to exploit temporal characteristics for segmentation purposes. Furthermore, the incorporation of a Multiscale Feature Extraction Module aims to grasp varied scale features within the UNet++. Through the amalgamation of our proposed methodology with data augmentation on the BUSI with GT dataset, an accuracy rate of 98.88%, specificity of 99.53%, precision of 95.34%, sensitivity of 91.20%, F1-score of 93.74, and Dice coefficient of 92.74% are achieved. These findings demonstrate competitiveness with cutting-edge techniques outlined in existing literature.

Multi-scale and Multi-path Cascaded Convolutional Network for Semantic Segmentation of Colorectal Polyps

Colorectal polyps are structural abnormalities of the gastrointestinal tract that can potentially become cancerous in some cases. The study introduces a novel framework for colorectal polyp segmentation named the Multi-Scale and Multi-Path Cascaded Convolution Network (MMCC-Net), aimed at addressing the limitations of existing models, such as inadequate spatial dependence representation and the absence of multi-level feature integration during the decoding stage by integrating multi-scale and multi-path cascaded convolutional techniques and enhances feature aggregation through dual attention modules, skip connections, and a feature enhancer. MMCC-Net achieves superior performance in identifying polyp areas at the pixel level. The Proposed MMCC-Net was tested across six public datasets and compared against eight SOTA models to demonstrate its efficiency in polyp segmentation. The MMCC-Net's performance shows Dice scores with confidence intervals ranging between (77.08, 77.56) and (94.19, 94.71) and Mean Intersection over Union (MIoU) scores with confidence intervals ranging from (72.20, 73.00) to (89.69, 90.53) on the six databases. These results highlight the model's potential as a powerful tool for accurate and efficient polyp segmentation, contributing to early detection and prevention strategies in colorectal cancer.

Full Field Digital Mammography Dataset from a Population Screening Program

Breast cancer presents the second largest cancer risk in the world to women. Early detection of cancer has been shown to be effective in reducing mortality. Population screening programs schedule regular mammography imaging for participants, promoting early detection. Currently, such screening programs require manual reading. False-positive errors in the reading process unnecessarily leads to costly follow-up and patient anxiety. Automated methods promise to provide more efficient, consistent and effective reading. To facilitate their development, a number of datasets have been created. With the aim of specifically targeting population screening programs, we introduce NL-Breast-Screening, a dataset from a Canadian provincial screening program. The dataset consists of 5997 mammography exams, each of which has four standard views and is biopsy-confirmed. Cases where radiologist reading was a false-positive are identified. NL-Breast is made publicly available as a new resource to promote advances in automation for population screening programs.

Research on Cervical Cancer p16/Ki-67 Immunohistochemical Dual-Staining Image Recognition Algorithm Based on YOLO

The p16/Ki-67 dual staining method is a new approach for cervical cancer screening with high sensitivity and specificity. However, there are issues of mis-detection and inaccurate recognition when the YOLOv5s algorithm is directly applied to dual-stained cell images. This paper Proposes a novel cervical cancer dual-stained image recognition (DSIR-YOLO) model based on an YOLOv5. By fusing the Swin-Transformer module, GAM attention mechanism, multi-scale feature fusion, and EIoU loss function, the detection performance is significantly improved, with mAP@0.5 and mAP@0.5:0.95 reaching 92.6% and 70.5%, respectively. Compared with YOLOv5s in five-fold cross-validation, the accuracy, recall, mAP@0.5, and mAP@0.5:0.95 of the improved algorithm are increased by 2.3%, 4.1%, 4.3%, and 8.0%, respectively, with smaller variances and higher stability. Compared with other detection algorithms, DSIR-YOLO in this paper sacrifices some performance requirements to improve the network recognition effect. In addition, the influence of dataset quality on the detection results is studied. By controlling the sealing property of pixels, scale difference, unlabelled cells, and diagonal annotation, the model detection accuracy, recall, mAP@0.5, and mAP@0.5:0.95 are improved by 13.3%, 15.3%, 18.3%, and 30.5%, respectively.