Sparser2Sparse: Single-shot Sparser-to-Sparse Learning for Spatial Transcriptomics Imputation with Natural Image Co-learning

Spatial transcriptomics (ST) has revolutionized biomedical research by enabling high resolution gene expression profiling within tissues. However, the high cost and scarcity of high resolution ST data remain significant challenges. We present Single-shot Sparser-to-Sparse (S2S-ST), a novel framework for accurate ST imputation that requires only a single and low-cost sparsely sampled ST dataset alongside widely available natural images for co-training. Our approach integrates three key innovations: (1) a sparser-to-sparse self-supervised learning strategy that leverages intrinsic spatial patterns in ST data, (2) cross-domain co-learning with natural images to enhance feature representation, and (3) a Cascaded Data Consistent Imputation Network (CDCIN) that iteratively refines predictions while preserving sampled gene data fidelity. Extensive experiments on diverse tissue types, including breast cancer, liver, and lymphoid tissue, demonstrate that our method outperforms state-of-the-art approaches in imputation accuracy. By enabling robust ST reconstruction from sparse inputs, our framework significantly reduces reliance on costly high resolution data, facilitating potential broader adoption in biomedical research and clinical applications.

AMA-SAM: Adversarial Multi-Domain Alignment of Segment Anything Model for High-Fidelity Histology Nuclei Segmentation

Accurate segmentation of cell nuclei in histopathology images is essential for numerous biomedical research and clinical applications. However, existing cell nucleus segmentation methods only consider a single dataset (i.e., primary domain), while neglecting to leverage supplementary data from diverse sources (i.e., auxiliary domains) to reduce overfitting and enhance the performance. Although incorporating multiple datasets could alleviate overfitting, it often exacerbates performance drops caused by domain shifts. In this work, we introduce Adversarial Multi-domain Alignment of Segment Anything Model (AMA-SAM) that extends the Segment Anything Model (SAM) to overcome these obstacles through two key innovations. First, we propose a Conditional Gradient Reversal Layer (CGRL), a multi-domain alignment module that harmonizes features from diverse domains to promote domain-invariant representation learning while preserving crucial discriminative features for the primary dataset. Second, we address SAM's inherent low-resolution output by designing a High-Resolution Decoder (HR-Decoder), which directly produces fine-grained segmentation maps in order to capture intricate nuclei boundaries in high-resolution histology images. To the best of our knowledge, this is the first attempt to adapt SAM for multi-dataset learning with application to histology nuclei segmentation. We validate our method on several publicly available datasets, demonstrating consistent and significant improvements over state-of-the-art approaches.

Teacher Agent: A Non-Knowledge Distillation Method for Rehearsal-based Video Incremental Learning

With the rise in popularity of video-based social media, new categories of videos are constantly being generated, creating an urgent need for robust incremental learning techniques for video understanding. One of the biggest challenges in this task is catastrophic forgetting, where the network tends to forget previously learned data while learning new categories. To overcome this issue, knowledge distillation is a widely used technique for rehearsal-based video incremental learning that involves transferring important information on similarities among different categories to enhance the student model. Therefore, it is preferable to have a strong teacher model to guide the students. However, the limited performance of the network itself and the occurrence of catastrophic forgetting can result in the teacher network making inaccurate predictions for some memory exemplars, ultimately limiting the student network's performance. Based on these observations, we propose a teacher agent capable of generating stable and accurate soft labels to replace the output of the teacher model. This method circumvents the problem of knowledge misleading caused by inaccurate predictions of the teacher model and avoids the computational overhead of loading the teacher model for knowledge distillation. Extensive experiments demonstrate the advantages of our method, yielding significant performance improvements while utilizing only half the resolution of video clips in the incremental phases as input compared to recent state-of-the-art methods. Moreover, our method surpasses the performance of joint training when employing four times the number of samples in episodic memory.