Multispectral photoacoustic tomography (PAT) is an imaging modality that utilizes the photoacoustic effect to achieve non-invasive and high-contrast imaging of internal tissues. However, the hardware cost and computational demand of a multispectral PAT system consisting of up to thousands of detectors are huge. To address this challenge, we propose an ultra-sparse spiral sampling strategy for multispectral PAT, which we named U3S-PAT. Our strategy employs a sparse ring-shaped transducer that, when switching excitation wavelengths, simultaneously rotates and translates. This creates a spiral scanning pattern with multispectral angle-interlaced sampling. To solve the highly ill-conditioned image reconstruction problem, we propose a self-supervised learning method that is able to introduce structural information shared during spiral scanning. We simulate the proposed U3S-PAT method on a commercial PAT system and conduct in vivo animal experiments to verify its performance. The results show that even with a sparse sampling rate as low as 1/30, our U3S-PAT strategy achieves similar reconstruction and spectral unmixing accuracy as non-spiral dense sampling. Given its ability to dramatically reduce the time required for three-dimensional multispectral scanning, our U3S-PAT strategy has the potential to perform volumetric molecular imaging of dynamic biological activities.
Photoacoustic tomography (PAT) is a promising imaging technique that can visualize the distribution of chromophores within biological tissue. However, the accuracy of PAT imaging is compromised by light fluence (LF), which hinders the quantification of light absorbers. Currently, model-based iterative methods are used for LF correction, but they require significant computational resources due to repeated LF estimation based on differential light transport models. To improve LF correction efficiency, we propose to use Fourier neural operator (FNO), a neural network specially designed for solving differential equations, to learn the forward projection of light transport in PAT. Trained using paired finite-element-based LF simulation data, our FNO model replaces the traditional computational heavy LF estimator during iterative correction, such that the correction procedure is significantly accelerated. Simulation and experimental results demonstrate that our method achieves comparable LF correction quality to traditional iterative methods while reducing the correction time by over 30 times.