Transcriptomic Models for Immunotherapy Response Prediction Show Limited Cross-cohort Generalisability

Immune checkpoint inhibitors (ICIs) have transformed cancer therapy; yet substantial proportion of patients exhibit intrinsic or acquired resistance, making accurate pre-treatment response prediction a critical unmet need. Transcriptomics-based biomarkers derived from bulk and single-cell RNA sequencing (scRNA-seq) offer a promising avenue for capturing tumour-immune interactions, yet the cross-cohort generalisability of existing prediction models remains unclear.We systematically benchmark nine state-of-the-art transcriptomic ICI response predictors, five bulk RNA-seq-based models (COMPASS, IRNet, NetBio, IKCScore, and TNBC-ICI) and four scRNA-seq-based models (PRECISE, DeepGeneX, Tres and scCURE), using publicly available independent datasets unseen during model development. Overall, predictive performance was modest: bulk RNA-seq models performed at or near chance level across most cohorts, while scRNA-seq models showed only marginal improvements. Pathway-level analyses revealed sparse and inconsistent biomarker signals across models. Although scRNA-seq-based predictors converged on immune-related programs such as allograft rejection, bulk RNA-seq-based models exhibited little reproducible overlap. PRECISE and NetBio identified the most coherent immune-related themes, whereas IRNet predominantly captured metabolic pathways weakly aligned with ICI biology. Together, these findings demonstrate the limited cross-cohort robustness and biological consistency of current transcriptomic ICI prediction models, underscoring the need for improved domain adaptation, standardised preprocessing, and biologically grounded model design.

Mamba-VA: A Mamba-based Approach for Continuous Emotion Recognition in Valence-Arousal Space

Continuous Emotion Recognition (CER) plays a crucial role in intelligent human-computer interaction, mental health monitoring, and autonomous driving. Emotion modeling based on the Valence-Arousal (VA) space enables a more nuanced representation of emotional states. However, existing methods still face challenges in handling long-term dependencies and capturing complex temporal dynamics. To address these issues, this paper proposes a novel emotion recognition model, Mamba-VA, which leverages the Mamba architecture to efficiently model sequential emotional variations in video frames. First, the model employs a Masked Autoencoder (MAE) to extract deep visual features from video frames, enhancing the robustness of temporal information. Then, a Temporal Convolutional Network (TCN) is utilized for temporal modeling to capture local temporal dependencies. Subsequently, Mamba is applied for long-sequence modeling, enabling the learning of global emotional trends. Finally, a fully connected (FC) layer performs regression analysis to predict continuous valence and arousal values. Experimental results on the Valence-Arousal (VA) Estimation task of the 8th competition on Affective Behavior Analysis in-the-wild (ABAW) demonstrate that the proposed model achieves valence and arousal scores of 0.5362 (0.5036) and 0.4310 (0.4119) on the validation (test) set, respectively, outperforming the baseline. The source code is available on GitHub:https://github.com/FreedomPuppy77/Charon.