Label-free Super-Resolution Microvessel Color Flow Imaging with Ultrasound

We present phase subtraction imaging (PSI), a new spatial-temporal beamforming method that enables micrometer level resolution imaging of microvessels in live animals without labels, which are microbubbles in ultrasound super-resolution imaging. Subtraction of relative phase differences between consecutive frames beamformed with mismatched apodizations is used in PSI to overcome the diffraction limit. We validated this method by imaging both the mouse brain and rabbit kidney using different ultrasound probes and scanning machines.

Grating lobe reduction in plane wave imaging with angular compounding using subtraction of coherent signals

Plane wave imaging (PWI) with angular compounding has gained in popularity over recent years because it provides high frame rates and good image properties. However, most linear arrays used in clinical practice have a pitch that is equal to than the wavelength of ultrasound. Hence, the presence of grating lobes is a concern for PWI using multiple transmit angles. The presence of grating lobes produces clutter in images and reduces the ability to observe tissue contrast. Techniques to reduce or eliminate the presence of grating lobes for PWI using multiple angles will result in improved image quality. Null subtraction imaging (NSI) is a nonlinear beamforming technique that has been explored for improving the lateral resolution of ultrasonic imaging. However, the apodization scheme used in NSI also eliminates or greatly reduces the presence of grating lobes. Imaging tasks using NSI were evaluated in simulations and physical experiments involving tissue-mimicking phantoms and rat tumors in vivo. Images created with NSI were compared with images created using traditional delay and sum (DAS) with Hann apodization and images created using a generalized coherence factor (GCF). NSI was observed to greatly reduce the presence of grating lobes in ultrasonic images, compared to DAS with Hann and GCF, while maintaining spatial resolution and contrast in the images. Therefore, NSI can provide a novel means of creating images using PWI with multiple steering angles on clinically available linear arrays while reducing the adverse effects associated with grating lobes.

Video-Streaming Biomedical Implants using Ultrasonic Waves for Communication

The use of wireless implanted medical devices (IMDs) is growing because they facilitate continuous monitoring of patients during normal activities, simplify medical procedures required for data retrieval and reduce the likelihood of infection associated with trailing wires. However, most of the state-of-the-art IMDs are passive and offline devices. One of the key obstacles to an active and online IMD is the infeasibility of real-time, high-quality video broadcast from the IMD. Such broadcast would help develop innovative devices such as a video-streaming capsule endoscopy (CE) pill with therapeutic intervention capabilities. State-of-the-art IMDs employ radio-frequency electromagnetic waves for information transmission. However, high attenuation of RF-EM waves in tissues and federal restrictions on the transmit power and operable bandwidth lead to fundamental performance constraints for IMDs employing RF links, and prevent achieving high data rates that could accomodate video broadcast. In this work, ultrasonic waves were used for video transmission and broadcast through biological tissues. The proposed proof-of-concept system was tested on a porcine intestine ex vivo and a rabbit in vivo. It was demonstrated that using a millimeter-sized, implanted biocompatible transducer operating at 1.1-1.2 MHz, it was possible to transmit endoscopic video with high resolution (1280 pixels by 720 pixels) through porcine intestine wrapped with bacon, and to broadcast standard definition (640 pixels by 480 pixels) video near real-time through rabbit abdomen in vivo. A media repository that includes experimental demonstrations and media files accompanies this paper. The accompanying media repository can be found at this link: https://bit.ly/3wuc7tk.