EAP4EMSIG -- Enhancing Event-Driven Microscopy for Microfluidic Single-Cell Analysis

Microfluidic Live-Cell Imaging yields data on microbial cell factories. However, continuous acquisition is challenging as high-throughput experiments often lack realtime insights, delaying responses to stochastic events. We introduce three components in the Experiment Automation Pipeline for Event-Driven Microscopy to Smart Microfluidic Single-Cell Analysis: a fast, accurate Deep Learning autofocusing method predicting the focus offset, an evaluation of real-time segmentation methods and a realtime data analysis dashboard. Our autofocusing achieves a Mean Absolute Error of 0.0226\textmu m with inference times below 50~ms. Among eleven Deep Learning segmentation methods, Cellpose~3 reached a Panoptic Quality of 93.58\%, while a distance-based method is fastest (121~ms, Panoptic Quality 93.02\%). All six Deep Learning Foundation Models were unsuitable for real-time segmentation.

Decision-Focused Fine-Tuning of Time Series Foundation Models for Dispatchable Feeder Optimization

Time series foundation models provide a universal solution for generating forecasts to support optimization problems in energy systems. Those foundation models are typically trained in a prediction-focused manner to maximize forecast quality. In contrast, decision-focused learning directly improves the resulting value of the forecast in downstream optimization rather than merely maximizing forecasting quality. The practical integration of forecast values into forecasting models is challenging, particularly when addressing complex applications with diverse instances, such as buildings. This becomes even more complicated when instances possess specific characteristics that require instance-specific, tailored predictions to increase the forecast value. To tackle this challenge, we use decision-focused fine-tuning within time series foundation models to offer a scalable and efficient solution for decision-focused learning applied to the dispatchable feeder optimization problem. To obtain more robust predictions for scarce building data, we use Moirai as a state-of-the-art foundation model, which offers robust and generalized results with few-shot parameter-efficient fine-tuning. Comparing the decision-focused fine-tuned Moirai with a state-of-the-art classical prediction-focused fine-tuning Morai, we observe an improvement of 9.45% in average total daily costs.

AutoPQ: Automating Quantile estimation from Point forecasts in the context of sustainability

Optimizing smart grid operations relies on critical decision-making informed by uncertainty quantification, making probabilistic forecasting a vital tool. Designing such forecasting models involves three key challenges: accurate and unbiased uncertainty quantification, workload reduction for data scientists during the design process, and limitation of the environmental impact of model training. In order to address these challenges, we introduce AutoPQ, a novel method designed to automate and optimize probabilistic forecasting for smart grid applications. AutoPQ enhances forecast uncertainty quantification by generating quantile forecasts from an existing point forecast by using a conditional Invertible Neural Network (cINN). AutoPQ also automates the selection of the underlying point forecasting method and the optimization of hyperparameters, ensuring that the best model and configuration is chosen for each application. For flexible adaptation to various performance needs and available computing power, AutoPQ comes with a default and an advanced configuration, making it suitable for a wide range of smart grid applications. Additionally, AutoPQ provides transparency regarding the electricity consumption required for performance improvements. We show that AutoPQ outperforms state-of-the-art probabilistic forecasting methods while effectively limiting computational effort and hence environmental impact. Additionally and in the context of sustainability, we quantify the electricity consumption required for performance improvements.

On autoregressive deep learning models for day-ahead wind power forecasting with irregular shutdowns due to redispatching

Renewable energies and their operation are becoming increasingly vital for the stability of electrical power grids since conventional power plants are progressively being displaced, and their contribution to redispatch interventions is thereby diminishing. In order to consider renewable energies like Wind Power (WP) for such interventions as a substitute, day-ahead forecasts are necessary to communicate their availability for redispatch planning. In this context, automated and scalable forecasting models are required for the deployment to thousands of locally-distributed onshore WP turbines. Furthermore, the irregular interventions into the WP generation capabilities due to redispatch shutdowns pose challenges in the design and operation of WP forecasting models. Since state-of-the-art forecasting methods consider past WP generation values alongside day-ahead weather forecasts, redispatch shutdowns may impact the forecast. Therefore, the present paper highlights these challenges and analyzes state-of-the-art forecasting methods on data sets with both regular and irregular shutdowns. Specifically, we compare the forecasting accuracy of three autoregressive Deep Learning (DL) methods to methods based on WP curve modeling. Interestingly, the latter achieve lower forecasting errors, have fewer requirements for data cleaning during modeling and operation while being computationally more efficient, suggesting their advantages in practical applications.

EAP4EMSIG -- Experiment Automation Pipeline for Event-Driven Microscopy to Smart Microfluidic Single-Cells Analysis

Microfluidic Live-Cell Imaging (MLCI) generates high-quality data that allows biotechnologists to study cellular growth dynamics in detail. However, obtaining these continuous data over extended periods is challenging, particularly in achieving accurate and consistent real-time event classification at the intersection of imaging and stochastic biology. To address this issue, we introduce the Experiment Automation Pipeline for Event-Driven Microscopy to Smart Microfluidic Single-Cells Analysis (EAP4EMSIG). In particular, we present initial zero-shot results from the real-time segmentation module of our approach. Our findings indicate that among four State-Of-The- Art (SOTA) segmentation methods evaluated, Omnipose delivers the highest Panoptic Quality (PQ) score of 0.9336, while Contour Proposal Network (CPN) achieves the fastest inference time of 185 ms with the second-highest PQ score of 0.8575. Furthermore, we observed that the vision foundation model Segment Anything is unsuitable for this particular use case.

Generating peak-aware pseudo-measurements for low-voltage feeders using metadata of distribution system operators

Distribution system operators (DSOs) must cope with new challenges such as the reconstruction of distribution grids along climate neutrality pathways or the ability to manage and control consumption and generation in the grid. In order to meet the challenges, measurements within the distribution grid often form the basis for DSOs. Hence, it is an urgent problem that measurement devices are not installed in many low-voltage (LV) grids. In order to overcome this problem, we present an approach to estimate pseudo-measurements for non-measured LV feeders based on the metadata of the respective feeder using regression models. The feeder metadata comprise information about the number of grid connection points, the installed power of consumers and producers, and billing data in the downstream LV grid. Additionally, we use weather data, calendar data and timestamp information as model features. The existing measurements are used as model target. We extensively evaluate the estimated pseudo-measurements on a large real-world dataset with 2,323 LV feeders characterized by both consumption and feed-in. For this purpose, we introduce peak metrics inspired by the BigDEAL challenge for the peak magnitude, timing and shape for both consumption and feed-in. As regression models, we use XGBoost, a multilayer perceptron (MLP) and a linear regression (LR). We observe that XGBoost and MLP outperform the LR. Furthermore, the results show that the approach adapts to different weather, calendar and timestamp conditions and produces realistic load curves based on the feeder metadata. In the future, the approach can be adapted to other grid levels like substation transformers and can supplement research fields like load modeling, state estimation and LV load forecasting.

Coverage Path Planning for Thermal Interface Materials

Thermal management of power electronics and Electronic Control Units is crucial in times of increasing power densities and limited assembly space. Electric and autonomous vehicles are a prominent application field. Thermal Interface Materials are used to transfer heat from a semiconductor to a heatsink. They are applied along a dispense path onto the semiconductor and spread over its entire surface once the heatsink is joined. To plan this application path, design engineers typically perform an iterative trial-and-error procedure of elaborate simulations and manual experiments. We propose a fully automated optimization approach, which clearly outperforms the current manual path planning and respects all relevant manufacturing constraints. An optimum dispense path increases the reliability of the thermal interface and makes the manufacturing more sustainable by reducing material waste. We show results on multiple real products from automotive series production, including an experimental validation on actual series manufacturing equipment.

Iterative Cluster Harvesting for Wafer Map Defect Patterns

Unsupervised clustering of wafer map defect patterns is challenging because the appearance of certain defect patterns varies significantly. This includes changing shape, location, density, and rotation of the defect area on the wafer. We present a harvesting approach, which can cluster even challenging defect patterns of wafer maps well. Our approach makes use of a well-known, three-step procedure: feature extraction, dimension reduction, and clustering. The novelty in our approach lies in repeating dimensionality reduction and clustering iteratively while filtering out one cluster per iteration according to its silhouette score. This method leads to an improvement of clustering performance in general and is especially useful for difficult defect patterns. The low computational effort allows for a quick assessment of large datasets and can be used to support manual labeling efforts. We benchmark against related approaches from the literature and show improved results on a real-world industrial dataset.

AI-based automated active learning for discovery of hidden dynamic processes: A use case in light microscopy

In the biomedical environment, experiments assessing dynamic processes are primarily performed by a human acquisition supervisor. Contemporary implementations of such experiments frequently aim to acquire a maximum number of relevant events from sometimes several hundred parallel, non-synchronous processes. Since in some high-throughput experiments, only one or a few instances of a given process can be observed simultaneously, a strategy for planning and executing an efficient acquisition paradigm is essential. To address this problem, we present two new methods in this paper. The first method, Encoded Dynamic Process (EDP), is Artificial Intelligence (AI)-based and represents dynamic processes so as to allow prediction of pseudo-time values from single still images. Second, with Experiment Automation Pipeline for Dynamic Processes (EAPDP), we present a Machine Learning Operations (MLOps)-based pipeline that uses the extracted knowledge from EDP to efficiently schedule acquisition in biomedical experiments for dynamic processes in practice. In a first experiment, we show that the pre-trained State-Of-The- Art (SOTA) object segmentation method Contour Proposal Networks (CPN) works reliably as a module of EAPDP to extract the relevant object for EDP from the acquired three-dimensional image stack.

MLOps for Scarce Image Data: A Use Case in Microscopic Image Analysis

Nowadays, Machine Learning (ML) is experiencing tremendous popularity that has never been seen before. The operationalization of ML models is governed by a set of concepts and methods referred to as Machine Learning Operations (MLOps). Nevertheless, researchers, as well as professionals, often focus more on the automation aspect and neglect the continuous deployment and monitoring aspects of MLOps. As a result, there is a lack of continuous learning through the flow of feedback from production to development, causing unexpected model deterioration over time due to concept drifts, particularly when dealing with scarce data. This work explores the complete application of MLOps in the context of scarce data analysis. The paper proposes a new holistic approach to enhance biomedical image analysis. Our method includes: a fingerprinting process that enables selecting the best models, datasets, and model development strategy relative to the image analysis task at hand; an automated model development stage; and a continuous deployment and monitoring process to ensure continuous learning. For preliminary results, we perform a proof of concept for fingerprinting in microscopic image datasets.