Adaptive coded illumination Fourier ptychography microscopy based on physical neural network

Fourier Ptychographic Microscopy (FPM) is a computational technique that achieves a large space-bandwidth product imaging. It addresses the challenge of balancing a large field of view and high resolution by fusing information from multiple images taken with varying illumination angles. Nevertheless, conventional FPM framework always suffers from long acquisition time and a heavy computational burden. In this paper, we propose a novel physical neural network that generates an adaptive illumination mode by incorporating temporally-encoded illumination modes as a distinct layer, aiming to improve the acquisition and calculation efficiency. Both simulations and experiments have been conducted to validate the feasibility and effectiveness of the proposed method. It is worth mentioning that, unlike previous works that obtain the intensity of a multiplexed illumination by post-combination of each sequentially illuminated and obtained low-resolution images, our experimental data is captured directly by turning on multiple LEDs with a coded illumination pattern. Our method has exhibited state-of-the-art performance in terms of both detail fidelity and imaging velocity when assessed through a multitude of evaluative aspects.

Single-shot quantitative differential phase contrast imaging combined with programmable polarization multiplexing illumination

We propose a single-shot quantitative differential phase contrast (DPC) method with polarization multiplexing illumination. In the illumination module of our system, the programmable LED array is divided into four quadrants and covered with polarizing films of four different polarization angles. We use a polarization camera with polarizers before the pixels in the imaging module. By matching the polarization angle between the polarizing films over the custom LED array and the polarizers in the camera, two sets of asymmetric illumination acquisition images can be calculated from a single-shot acquisition image. Combined with the phase transfer function, we can calculate the quantitative phase of the sample. We present the design, implementation, and experimental image data demonstrating the ability of our method to obtain quantitative phase images of the phase resolution target, as well as Hela cells.

Robust full-pose-parameter estimation for the LED array in Fourier ptychographic microscopy

Fourier ptychographic microscopy (FPM) can achieve quantitative phase imaging with a large space-bandwidth product by synthesizing a set of low-resolution intensity images captured under angularly varying illuminations. Determining accurate illumination angles is critical because the consistency between actual systematic parameters and those used in the recovery algorithm is essential for high-quality imaging. This paper presents a full-pose-parameter and physics-based method for calibrating illumination angles. Using a physics-based model constructed with general knowledge of the employed microscope and the brightfield-to-darkfield boundaries inside captured images, we can solve for the full-pose parameters of misplaced LED array, which consist of the distance between the sample and the LED array, two orthogonal lateral shifts, one in-plane rotation angle, and two tilt angles, to correct illumination angles precisely. The feasibility and effectiveness of the proposed method for recovering random or remarkable pose parameters have been demonstrated by both qualitative and quantitative experiments. Due to the completeness of the pose parameters, the clarity of the physical model, and the high robustness for arbitrary misalignments, our method can significantly facilitate the design, implementation, and application of concise and robust FPM platforms.

Pose correction scheme for camera-scanning Fourier ptychography based on camera calibration and homography transform

Fourier ptychography (FP), as a computational imaging method, is a powerful tool to improve imaging resolution. Camera-scanning Fourier ptychography extends the application of FP from micro to macro creatively. Due to the non-ideal scanning of the camera driven by the mechanical translation stage, the pose error of the camera occurs, greatly degrading the reconstruction quality, while a precise translation stage is expensive and not suitable for wide-range imaging. Here, to improve the imaging performance of camera-scanning Fourier ptychography, we propose a pose correction scheme based on camera calibration and homography transform approaches. The scheme realizes the accurate alignment of data set and location error correction in the frequency domain. Simulation and experimental results demonstrate this method can optimize the reconstruction results and realize high-quality imaging effectively. Combined with the feature recognition algorithm, the scheme provides the possibility for applying FP in remote sensing imaging and space imaging.

Parallel Fourier Ptychography reconstruction

Fourier ptychography has attracted a wide range of focus for its ability of large space-bandwidth-produce, and quantative phase measurement. It is a typical computational imaging technique which refers to optimizing both the imaging hardware and reconstruction algorithms simultaneously. The data redundancy and inverse problem algorithms are the sources of FPM's excellent performance. But at the same time, this large amount of data processing and complex algorithms also greatly reduce the imaging speed. In this article, we propose a parallel Fourier ptychography reconstruction framework consisting of three levels of parallel computing parts and implemented it with both central processing unit (CPU) and compute unified device architecture (CUDA) platform. In the conventional FPM reconstruction framework, the sample image is divided into multiple sub-regions for separately processing because the illumination angles for different subregions are varied for the same LED and different subregions contain different defocus distances due to the non-planar distribution or non-ideal posture of biological sample. We first build a parallel computing sub-framework in spatial domain based on the above-mentioned characteristics. And then, by utilizing the sequential characteristics of different spectrum regions to update, a parallel computing sub-framework in the spectrum domain is carried out in our scheme. The feasibility of the proposed parallel FPM reconstruction framework is verified with different experimental results acquired with the system we built.

Fourier ptychography multi-parameter neural network with composite physical priori optimization

Fourier ptychography microscopy(FP) is a recently developed computational imaging approach for microscopic super-resolution imaging. By turning on each light-emitting-diode (LED) located on different position on the LED array sequentially and acquiring the corresponding images that contain different spatial frequency components, high spatial resolution and quantitative phase imaging can be achieved in the case of large field-of-view. Nevertheless, FPM has high requirements for the system construction and data acquisition processes, such as precise LEDs position, accurate focusing and appropriate exposure time, which brings many limitations to its practical applications. In this paper, inspired by artificial neural network, we propose a Fourier ptychography multi-parameter neural network (FPMN) with composite physical prior optimization. A hybrid parameter determination strategy combining physical imaging model and data-driven network training is proposed to recover the multi layers of the network corresponding to different physical parameters, including sample complex function, system pupil function, defocus distance, LED array position deviation and illumination intensity fluctuation, etc. Among these parameters, LED array position deviation is recovered based on the features of brightfield to darkfield transition low-resolution images while the others are recovered in the process of training of the neural network. The feasibility and effectiveness of FPMN are verified through simulations and actual experiments. Therefore FPMN can evidently reduce the requirement for practical applications of FPM.