FM2S: Self-Supervised Fluorescence Microscopy Denoising With Single Noisy Image

Fluorescence microscopy has significantly advanced biological research by visualizing detailed cellular structures and biological processes. However, such image denoising task often faces challenges due to difficulty in precisely modeling the inherent noise and acquiring clean images for training, which constrains most existing methods. In this paper, we propose an efficient self-supervised denoiser Fluorescence Micrograph to Self (FM2S), enabling a high-quality denoised result with a single noisy image. Our method introduces an adaptive global-local Noise Addition module for data augmentation, addressing generalization problems caused by discrepancies between synthetic and real-world noise. We then train a two-layer neural network to learn the mapping from the noise-added image to the filtered image, achieving a balance between noise removal and computational efficiency. Experimental results demonstrate that FM2S excels in various microscope types and noise levels in terms of denoising effects and time consumption, obtaining an average PSNR improvement of around 6 dB over the original noisy image in a few seconds. The code is available at https://github.com/Danielement321/FM2S.