CLIN-LLM: A Safety-Constrained Hybrid Framework for Clinical Diagnosis and Treatment Generation

Accurate symptom-to-disease classification and clinically grounded treatment recommendations remain challenging, particularly in heterogeneous patient settings with high diagnostic risk. Existing large language model (LLM)-based systems often lack medical grounding and fail to quantify uncertainty, resulting in unsafe outputs. We propose CLIN-LLM, a safety-constrained hybrid pipeline that integrates multimodal patient encoding, uncertainty-calibrated disease classification, and retrieval-augmented treatment generation. The framework fine-tunes BioBERT on 1,200 clinical cases from the Symptom2Disease dataset and incorporates Focal Loss with Monte Carlo Dropout to enable confidence-aware predictions from free-text symptoms and structured vitals. Low-certainty cases (18%) are automatically flagged for expert review, ensuring human oversight. For treatment generation, CLIN-LLM employs Biomedical Sentence-BERT to retrieve top-k relevant dialogues from the 260,000-sample MedDialog corpus. The retrieved evidence and patient context are fed into a fine-tuned FLAN-T5 model for personalized treatment generation, followed by post-processing with RxNorm for antibiotic stewardship and drug-drug interaction (DDI) screening. CLIN-LLM achieves 98% accuracy and F1 score, outperforming ClinicalBERT by 7.1% (p < 0.001), with 78% top-5 retrieval precision and a clinician-rated validity of 4.2 out of 5. Unsafe antibiotic suggestions are reduced by 67% compared to GPT-5. These results demonstrate CLIN-LLM's robustness, interpretability, and clinical safety alignment. The proposed system provides a deployable, human-in-the-loop decision support framework for resource-limited healthcare environments. Future work includes integrating imaging and lab data, multilingual extensions, and clinical trial validation.

LLM4Cell: A Survey of Large Language and Agentic Models for Single-Cell Biology

Large language models (LLMs) and emerging agentic frameworks are beginning to transform single-cell biology by enabling natural-language reasoning, generative annotation, and multimodal data integration. However, progress remains fragmented across data modalities, architectures, and evaluation standards. LLM4Cell presents the first unified survey of 58 foundation and agentic models developed for single-cell research, spanning RNA, ATAC, multi-omic, and spatial modalities. We categorize these methods into five families-foundation, text-bridge, spatial, multimodal, epigenomic, and agentic-and map them to eight key analytical tasks including annotation, trajectory and perturbation modeling, and drug-response prediction. Drawing on over 40 public datasets, we analyze benchmark suitability, data diversity, and ethical or scalability constraints, and evaluate models across 10 domain dimensions covering biological grounding, multi-omics alignment, fairness, privacy, and explainability. By linking datasets, models, and evaluation domains, LLM4Cell provides the first integrated view of language-driven single-cell intelligence and outlines open challenges in interpretability, standardization, and trustworthy model development.

Exploring Gene Regulatory Interaction Networks and predicting therapeutic molecules for Hypopharyngeal Cancer and EGFR-mutated lung adenocarcinoma

With the advent of Information technology, the Bioinformatics research field is becoming increasingly attractive to researchers and academicians. The recent development of various Bioinformatics toolkits has facilitated the rapid processing and analysis of vast quantities of biological data for human perception. Most studies focus on locating two connected diseases and making some observations to construct diverse gene regulatory interaction networks, a forerunner to general drug design for curing illness. For instance, Hypopharyngeal cancer is a disease that is associated with EGFR-mutated lung adenocarcinoma. In this study, we select EGFR-mutated lung adenocarcinoma and Hypopharyngeal cancer by finding the Lung metastases in hypopharyngeal cancer. To conduct this study, we collect Mircorarray datasets from GEO (Gene Expression Omnibus), an online database controlled by NCBI. Differentially expressed genes, common genes, and hub genes between the selected two diseases are detected for the succeeding move. Our research findings have suggested common therapeutic molecules for the selected diseases based on 10 hub genes with the highest interactions according to the degree topology method and the maximum clique centrality (MCC). Our suggested therapeutic molecules will be fruitful for patients with those two diseases simultaneously.