Median2Median: Zero-shot Suppression of Structured Noise in Images

Image denoising is a fundamental problem in computer vision and medical imaging. However, real-world images are often degraded by structured noise with strong anisotropic correlations that existing methods struggle to remove. Most data-driven approaches rely on large datasets with high-quality labels and still suffer from limited generalizability, whereas existing zero-shot methods avoid this limitation but remain effective only for independent and identically distributed (i.i.d.) noise. To address this gap, we propose Median2Median (M2M), a zero-shot denoising framework designed for structured noise. M2M introduces a novel sampling strategy that generates pseudo-independent sub-image pairs from a single noisy input. This strategy leverages directional interpolation and generalized median filtering to adaptively exclude values distorted by structured artifacts. To further enlarge the effective sampling space and eliminate systematic bias, a randomized assignment strategy is employed, ensuring that the sampled sub-image pairs are suitable for Noise2Noise training. In our realistic simulation studies, M2M performs on par with state-of-the-art zero-shot methods under i.i.d. noise, while consistently outperforming them under correlated noise. These findings establish M2M as an efficient, data-free solution for structured noise suppression and mark the first step toward effective zero-shot denoising beyond the strict i.i.d. assumption.

Machine Learning-Based Genomic Linguistic Analysis (Gene Sequence Feature Learning): A Case Study on Predicting Heavy Metal Response Genes in Rice

This study explores the application of machine learning-based genetic linguistics for identifying heavy metal response genes in rice (Oryza sativa). By integrating convolutional neural networks and random forest algorithms, we developed a hybrid model capable of extracting and learning meaningful features from gene sequences, such as k-mer frequencies and physicochemical properties. The model was trained and tested on datasets of genes, achieving high predictive performance (precision: 0.89, F1-score: 0.82). RNA-seq and qRT-PCR experiments conducted on rice leaves which exposed to Hg0, revealed differential expression of genes associated with heavy metal responses, which validated the model's predictions. Co-expression network analysis identified 103 related genes, and a literature review indicated that these genes are highly likely to be involved in heavy metal-related biological processes. By integrating and comparing the analysis results with those of differentially expressed genes (DEGs), the validity of the new machine learning method was further demonstrated. This study highlights the efficacy of combining machine learning with genetic linguistics for large-scale gene prediction. It demonstrates a cost-effective and efficient approach for uncovering molecular mechanisms underlying heavy metal responses, with potential applications in developing stress-tolerant crop varieties.