Correlation Clustering of Organoid Images

In biological and medical research, scientists now routinely acquire microscopy images of hundreds of morphologically heterogeneous organoids and are then faced with the task of finding patterns in the image collection, i.e., subsets of organoids that appear similar and potentially represent the same morphological class. We adopt models and algorithms for correlating organoid images, i.e., for quantifying the similarity in appearance and geometry of the organoids they depict, and for clustering organoid images by consolidating conflicting correlations. For correlating organoid images, we adopt and compare two alternatives, a partial quadratic assignment problem and a twin network. For clustering organoid images, we employ the correlation clustering problem. Empirically, we learn the parameters of these models, infer a clustering of organoid images, and quantify the accuracy of the inferred clusters, with respect to a training set and a test set we contribute of state-of-the-art light microscopy images of organoids clustered manually by biologists.

A Graph Multi-separator Problem for Image Segmentation

We propose a novel abstraction of the image segmentation task in the form of a combinatorial optimization problem that we call the multi-separator problem. Feasible solutions indicate for every pixel whether it belongs to a segment or a segment separator, and indicate for pairs of pixels whether or not the pixels belong to the same segment. This is in contrast to the closely related lifted multicut problem where every pixel is associated to a segment and no pixel explicitly represents a separating structure. While the multi-separator problem is NP-hard, we identify two special cases for which it can be solved efficiently. Moreover, we define two local search algorithms for the general case and demonstrate their effectiveness in segmenting simulated volume images of foam cells and filaments.