ACE-HetEM for ab initio Heterogenous Cryo-EM 3D Reconstruction

Due to the extremely low signal-to-noise ratio (SNR) and unknown poses (projection angles and image translation) in cryo-EM experiments, reconstructing 3D structures from 2D images is very challenging. On top of these challenges, heterogeneous cryo-EM reconstruction also has an additional requirement: conformation classification. An emerging solution to this problem is called amortized inference, implemented using the autoencoder architecture or its variants. Instead of searching for the correct image-to-pose/conformation mapping for every image in the dataset as in non-amortized methods, amortized inference only needs to train an encoder that maps images to appropriate latent spaces representing poses or conformations. Unfortunately, standard amortized-inference-based methods with entangled latent spaces have difficulty learning the distribution of conformations and poses from cryo-EM images. In this paper, we propose an unsupervised deep learning architecture called "ACE-HetEM" based on amortized inference. To explicitly enforce the disentanglement of conformation classifications and pose estimations, we designed two alternating training tasks in our method: image-to-image task and pose-to-pose task. Results on simulated datasets show that ACE-HetEM has comparable accuracy in pose estimation and produces even better reconstruction resolution than non-amortized methods. Furthermore, we show that ACE-HetEM is also applicable to real experimental datasets.

VecFontSDF: Learning to Reconstruct and Synthesize High-quality Vector Fonts via Signed Distance Functions

Font design is of vital importance in the digital content design and modern printing industry. Developing algorithms capable of automatically synthesizing vector fonts can significantly facilitate the font design process. However, existing methods mainly concentrate on raster image generation, and only a few approaches can directly synthesize vector fonts. This paper proposes an end-to-end trainable method, VecFontSDF, to reconstruct and synthesize high-quality vector fonts using signed distance functions (SDFs). Specifically, based on the proposed SDF-based implicit shape representation, VecFontSDF learns to model each glyph as shape primitives enclosed by several parabolic curves, which can be precisely converted to quadratic B\'ezier curves that are widely used in vector font products. In this manner, most image generation methods can be easily extended to synthesize vector fonts. Qualitative and quantitative experiments conducted on a publicly-available dataset demonstrate that our method obtains high-quality results on several tasks, including vector font reconstruction, interpolation, and few-shot vector font synthesis, markedly outperforming the state of the art.