A Methodological Framework for Measuring Spatial Labeling Similarity

Spatial labeling assigns labels to specific spatial locations to characterize their spatial properties and relationships, with broad applications in scientific research and practice. Measuring the similarity between two spatial labelings is essential for understanding their differences and the contributing factors, such as changes in location properties or labeling methods. An adequate and unbiased measurement of spatial labeling similarity should consider the number of matched labels (label agreement), the topology of spatial label distribution, and the heterogeneous impacts of mismatched labels. However, existing methods often fail to account for all these aspects. To address this gap, we propose a methodological framework to guide the development of methods that meet these requirements. Given two spatial labelings, the framework transforms them into graphs based on location organization, labels, and attributes (e.g., location significance). The distributions of their graph attributes are then extracted, enabling an efficient computation of distributional discrepancy to reflect the dissimilarity level between the two labelings. We further provide a concrete implementation of this framework, termed Spatial Labeling Analogy Metric (SLAM), along with an analysis of its theoretical foundation, for evaluating spatial labeling results in spatial transcriptomics (ST) \textit{as per} their similarity with ground truth labeling. Through a series of carefully designed experimental cases involving both simulated and real ST data, we demonstrate that SLAM provides a comprehensive and accurate reflection of labeling quality compared to other well-established evaluation metrics. Our code is available at https://github.com/YihDu/SLAM.

Domain Adaptive and Fine-grained Anomaly Detection for Single-cell Sequencing Data and Beyond

Fined-grained anomalous cell detection from affected tissues is critical for clinical diagnosis and pathological research. Single-cell sequencing data provide unprecedented opportunities for this task. However, current anomaly detection methods struggle to handle domain shifts prevalent in multi-sample and multi-domain single-cell sequencing data, leading to suboptimal performance. Moreover, these methods fall short of distinguishing anomalous cells into pathologically distinct subtypes. In response, we propose ACSleuth, a novel, reconstruction deviation-guided generative framework that integrates the detection, domain adaptation, and fine-grained annotating of anomalous cells into a methodologically cohesive workflow. Notably, we present the first theoretical analysis of using reconstruction deviations output by generative models for anomaly detection in lieu of domain shifts. This analysis informs us to develop a novel and superior maximum mean discrepancy-based anomaly scorer in ACSleuth. Extensive benchmarks over various single-cell data and other types of tabular data demonstrate ACSleuth's superiority over the state-of-the-art methods in identifying and subtyping anomalies in multi-sample and multi-domain contexts. Our code is available at https://github.com/Catchxu/ACsleuth.

Spatio-Temporal Bi-directional Cross-frame Memory for Distractor Filtering Point Cloud Single Object Tracking

3D single object tracking within LIDAR point clouds is a pivotal task in computer vision, with profound implications for autonomous driving and robotics. However, existing methods, which depend solely on appearance matching via Siamese networks or utilize motion information from successive frames, encounter significant challenges. Issues such as similar objects nearby or occlusions can result in tracker drift. To mitigate these challenges, we design an innovative spatio-temporal bi-directional cross-frame distractor filtering tracker, named STMD-Tracker. Our first step involves the creation of a 4D multi-frame spatio-temporal graph convolution backbone. This design separates KNN graph spatial embedding and incorporates 1D temporal convolution, effectively capturing temporal fluctuations and spatio-temporal information. Subsequently, we devise a novel bi-directional cross-frame memory procedure. This integrates future and synthetic past frame memory to enhance the current memory, thereby improving the accuracy of iteration-based tracking. This iterative memory update mechanism allows our tracker to dynamically compensate for information in the current frame, effectively reducing tracker drift. Lastly, we construct spatially reliable Gaussian masks on the fused features to eliminate distractor points. This is further supplemented by an object-aware sampling strategy, which bolsters the efficiency and precision of object localization, thereby reducing tracking errors caused by distractors. Our extensive experiments on KITTI, NuScenes and Waymo datasets demonstrate that our approach significantly surpasses the current state-of-the-art methods.