GastroDL-Fusion: A Dual-Modal Deep Learning Framework Integrating Protein-Ligand Complexes and Gene Sequences for Gastrointestinal Disease Drug Discovery

Accurate prediction of protein-ligand binding affinity plays a pivotal role in accelerating the discovery of novel drugs and vaccines, particularly for gastrointestinal (GI) diseases such as gastric ulcers, Crohn's disease, and ulcerative colitis. Traditional computational models often rely on structural information alone and thus fail to capture the genetic determinants that influence disease mechanisms and therapeutic responses. To address this gap, we propose GastroDL-Fusion, a dual-modal deep learning framework that integrates protein-ligand complex data with disease-associated gene sequence information for drug and vaccine development. In our approach, protein-ligand complexes are represented as molecular graphs and modeled using a Graph Isomorphism Network (GIN), while gene sequences are encoded into biologically meaningful embeddings via a pre-trained Transformer (ProtBERT/ESM). These complementary modalities are fused through a multi-layer perceptron to enable robust cross-modal interaction learning. We evaluate the model on benchmark datasets of GI disease-related targets, demonstrating that GastroDL-Fusion significantly improves predictive performance over conventional methods. Specifically, the model achieves a mean absolute error (MAE) of 1.12 and a root mean square error (RMSE) of 1.75, outperforming CNN, BiLSTM, GIN, and Transformer-only baselines. These results confirm that incorporating both structural and genetic features yields more accurate predictions of binding affinities, providing a reliable computational tool for accelerating the design of targeted therapies and vaccines in the context of gastrointestinal diseases.

Machine Learning Algorithm for Noise Reduction and Disease-Causing Gene Feature Extraction in Gene Sequencing Data

In this study, we propose a machine learning-based method for noise reduction and disease-causing gene feature extraction in gene sequencing DeepSeqDenoise algorithm combines CNN and RNN to effectively remove the sequencing noise, and improves the signal-to-noise ratio by 9.4 dB. We screened 17 key features by feature engineering, and constructed an integrated learning model to predict disease-causing genes with 94.3% accuracy. We successfully identified 57 new candidate disease-causing genes in a cardiovascular disease cohort validation, and detected 3 missed variants in clinical applications. The method significantly outperforms existing tools and provides strong support for accurate diagnosis of genetic diseases.