Zebrafish Counting Using Event Stream Data

Zebrafish share a high degree of homology with human genes and are commonly used as model organism in biomedical research. For medical laboratories, counting zebrafish is a daily task. Due to the tiny size of zebrafish, manual visual counting is challenging. Existing counting methods are either not applicable to small fishes or have too many limitations. The paper proposed a zebrafish counting algorithm based on the event stream data. Firstly, an event camera is applied for data acquisition. Secondly, camera calibration and image fusion were preformed successively. Then, the trajectory information was used to improve the counting accuracy. Finally, the counting results were averaged over an empirical of period and rounded up to get the final results. To evaluate the accuracy of the algorithm, 20 zebrafish were put in a four-liter breeding tank. Among 100 counting trials, the average accuracy reached 97.95%. As compared with traditional algorithms, the proposed one offers a simpler implementation and achieves higher accuracy.