Self-learned representation-guided latent diffusion model for breast cancer classification in deep ultraviolet whole surface images

Breast-Conserving Surgery (BCS) requires precise intraoperative margin assessment to preserve healthy tissue. Deep Ultraviolet Fluorescence Scanning Microscopy (DUV-FSM) offers rapid, high-resolution surface imaging for this purpose; however, the scarcity of annotated DUV data hinders the training of robust deep learning models. To address this, we propose an Self-Supervised Learning (SSL)-guided Latent Diffusion Model (LDM) to generate high-quality synthetic training patches. By guiding the LDM with embeddings from a fine-tuned DINO teacher, we inject rich semantic details of cellular structures into the synthetic data. We combine real and synthetic patches to fine-tune a Vision Transformer (ViT), utilizing patch prediction aggregation for WSI-level classification. Experiments using 5-fold cross-validation demonstrate that our method achieves 96.47 % accuracy and reduces the FID score to 45.72, significantly outperforming class-conditioned baselines.

Breast Cancer Classification in Deep Ultraviolet Fluorescence Images Using a Patch-Level Vision Transformer Framework

Breast-conserving surgery (BCS) aims to completely remove malignant lesions while maximizing healthy tissue preservation. Intraoperative margin assessment is essential to achieve a balance between thorough cancer resection and tissue conservation. A deep ultraviolet fluorescence scanning microscope (DUV-FSM) enables rapid acquisition of whole surface images (WSIs) for excised tissue, providing contrast between malignant and normal tissues. However, breast cancer classification with DUV WSIs is challenged by high resolutions and complex histopathological features. This study introduces a DUV WSI classification framework using a patch-level vision transformer (ViT) model, capturing local and global features. Grad-CAM++ saliency weighting highlights relevant spatial regions, enhances result interpretability, and improves diagnostic accuracy for benign and malignant tissue classification. A comprehensive 5-fold cross-validation demonstrates the proposed approach significantly outperforms conventional deep learning methods, achieving a classification accuracy of 98.33%.