We present an approach to leaf level segmentation of images of Arabidopsis thaliana plants based upon detected edges. We introduce a novel approach to edge classification, which forms an important part of a method to both count the leaves and establish the leaf area of a growing plant from images obtained in a high-throughput phenotyping system. Our technique uses a relatively shallow convolutional neural network to classify image edges as background, plant edge, leaf-on-leaf edge or internal leaf noise. The edges themselves were found using the Canny edge detector and the classified edges can be used with simple image processing techniques to generate a region-based segmentation in which the leaves are distinct. This approach is strong at distinguishing occluding pairs of leaves where one leaf is largely hidden, a situation which has proved troublesome for plant image analysis systems in the past. In addition, we introduce the publicly available plant image dataset that was used for this work.
We describe a new measure for the evaluation of region level segmentation of objects, as applied to evaluating the accuracy of leaf-level segmentation of plant images. The proposed approach enforces the rule that a region (e.g. a leaf) in either the image being evaluated or the ground truth image evaluated against can be mapped to no more than one region in the other image. We call this measure the subset-matched Jaccard index.