In situ Biological Particle Analyzer based on Digital Inline Holography

Obtaining in situ measurements of biological microparticles is crucial for both scientific research and numerous industrial applications (e.g., early detection of harmful algal blooms, monitoring yeast during fermentation). However, existing methods are limited to offer timely diagnostics of these particles with sufficient accuracy and information. Here, we introduce a novel method for real-time, in situ analysis using machine learning assisted digital inline holography (DIH). Our machine learning model uses a customized YOLO v5 architecture specialized for the detection and classification of small biological particles. We demonstrate the effectiveness of our method in the analysis of 10 plankton species with equivalent high accuracy and significantly reduced processing time compared to previous methods. We also applied our method to differentiate yeast cells under four metabolic states and from two strains. Our results show that the proposed method can accurately detect and differentiate cellular and subcellular features related to metabolic states and strains. This study demonstrates the potential of machine learning driven DIH approach as a sensitive and versatile diagnostic tool for real-time, in situ analysis of both biotic and abiotic particles. This method can be readily deployed in a distributive manner for scientific research and manufacturing on an industrial scale.

Data-driven yaw misalignment correction for utility-scale wind turbines

In recent years, wind turbine yaw misalignment that tends to degrade the turbine power production and impact the blade fatigue loads raises more attention along with the rapid development of large-scale wind turbines. The state-of-the-art correction methods require additional instruments such as LiDAR to provide the ground truths and are not suitable for long-term operation and large-scale implementation due to the high costs. In the present study, we propose a framework that enables the effective and efficient detection and correction of static and dynamic yaw errors by using only turbine SCADA data, suitable for a low-cost regular inspection for large-scale wind farms in onshore, coastal, and offshore sites. This framework includes a short-period data collection of the turbine operating under multiple static yaw errors, a data mining correction for the static yaw error, and ultra-short-term dynamic yaw error forecasts with machine learning algorithms. Three regression algorithms, i.e., linear, support vector machine, and random forest, and a hybrid model based on the average prediction of the three, have been tested for dynamic yaw error prediction and compared using the field measurement data from a 2.5 MW turbine. For the data collected in the present study, the hybrid method shows the best performance and can reduce total yaw error by up to 85% (on average of 71%) compared to the cases without static and dynamic yaw error corrections. In addition, we have tested the transferability of the proposed method in the application of detecting other static and dynamic yaw errors.

Design and Experiments with a Robot-Driven Underwater Holographic Microscope for Low-Cost In Situ Particle Measurements

Microscopic analysis of micro particles in situ in diverse water environments is necessary for monitoring water quality and localizing contamination sources. Conventional sensors such as optical microscopes and fluorometers often require complex sample preparation, are restricted to small sample volumes, and are unable to simultaneously capture all pertinent details of a sample such as particle size, shape, concentration, and three dimensional motion. In this article we propose a novel and cost-effective robotic system for mobile microscopic analysis of particles in situ at various depths which are fully controlled by the robot system itself. A miniature underwater digital in-line holographic microscope (DIHM) performs high resolution imaging of microparticles (e.g., algae cells, plastic debris, sediments) while movement allows measurement of particle distributions covering a large area of water. The main contribution of this work is the creation of a low-cost, comprehensive, and small underwater robotic holographic microscope that can assist in a variety of tasks in environmental monitoring and overall assessment of water quality such as contaminant detection and localization. The resulting system provides some unique capabilities such as expanded and systematic coverage of large bodies of water at a low cost. Several challenges such as the trade-off between image quality and cost are addressed to satisfy the aforementioned goals.