To safely deploy deep learning models in the clinic, a quality assurance framework is needed for routine or continuous monitoring of input-domain shift and the models' performance without ground truth contours. In this work, cardiac substructure segmentation was used as an example task to establish a QA framework. A benchmark dataset consisting of Computed Tomography (CT) images along with manual cardiac delineations of 241 patients were collected, including one 'common' image domain and five 'uncommon' domains. Segmentation models were tested on the benchmark dataset for an initial evaluation of model capacity and limitations. An image domain shift detector was developed by utilizing a trained Denoising autoencoder (DAE) and two hand-engineered features. Another Variational Autoencoder (VAE) was also trained to estimate the shape quality of the auto-segmentation results. Using the extracted features from the image/segmentation pair as inputs, a regression model was trained to predict the per-patient segmentation accuracy, measured by Dice coefficient similarity (DSC). The framework was tested across 19 segmentation models to evaluate the generalizability of the entire framework. As results, the predicted DSC of regression models achieved a mean absolute error (MAE) ranging from 0.036 to 0.046 with an averaged MAE of 0.041. When tested on the benchmark dataset, the performances of all segmentation models were not significantly affected by scanning parameters: FOV, slice thickness and reconstructions kernels. For input images with Poisson noise, CNN-based segmentation models demonstrated a decreased DSC ranging from 0.07 to 0.41, while the transformer-based model was not significantly affected.