Intraoperative perfusion assessment by continuous, low-latency hyperspectral light-field imaging: development, methodology, and clinical application

Accurate assessment of tissue perfusion is crucial in visceral surgery, especially during anastomosis. Currently, subjective visual judgment is commonly employed in clinical settings. Hyperspectral imaging (HSI) offers a non-invasive, quantitative alternative. However, HSI imaging lacks continuous integration into the clinical workflow. This study presents a hyperspectral light field system for intraoperative tissue oxygen saturation (SO2) analysis and visualization. We present a correlation method for determining SO2 saturation with low computational demands. We demonstrate clinical application, with our results aligning with the perfusion boundaries determined by the surgeon. We perform and compare continuous perfusion analysis using two hyperspectral cameras (Cubert S5, Cubert X20), achieving processing times of < 170 ms and < 400 ms, respectively. We discuss camera characteristics, system parameters, and the suitability for clinical use and real-time applications.

Automatic Tissue Differentiation in Parotidectomy using Hyperspectral Imaging

In head and neck surgery, continuous intraoperative tissue differentiation is of great importance to avoid injury to sensitive structures such as nerves and vessels. Hyperspectral imaging (HSI) with neural network analysis could support the surgeon in tissue differentiation. A 3D Convolutional Neural Network with hyperspectral data in the range of $400-1000$ nm is used in this work. The acquisition system consisted of two multispectral snapshot cameras creating a stereo-HSI-system. For the analysis, 27 images with annotations of glandular tissue, nerve, muscle, skin and vein in 18 patients undergoing parotidectomy are included. Three patients are removed for evaluation following the leave-one-subject-out principle. The remaining images are used for training, with the data randomly divided into a training group and a validation group. In the validation, an overall accuracy of $98.7\%$ is achieved, indicating robust training. In the evaluation on the excluded patients, an overall accuracy of $83.4\%$ has been achieved showing good detection and identification abilities. The results clearly show that it is possible to achieve robust intraoperative tissue differentiation using hyperspectral imaging. Especially the high sensitivity in parotid or nerve tissue is of clinical importance. It is interesting to note that vein was often confused with muscle. This requires further analysis and shows that a very good and comprehensive data basis is essential. This is a major challenge, especially in surgery.

Synthetic Generation of Dermatoscopic Images with GAN and Closed-Form Factorization

In the realm of dermatological diagnoses, where the analysis of dermatoscopic and microscopic skin lesion images is pivotal for the accurate and early detection of various medical conditions, the costs associated with creating diverse and high-quality annotated datasets have hampered the accuracy and generalizability of machine learning models. We propose an innovative unsupervised augmentation solution that harnesses Generative Adversarial Network (GAN) based models and associated techniques over their latent space to generate controlled semiautomatically-discovered semantic variations in dermatoscopic images. We created synthetic images to incorporate the semantic variations and augmented the training data with these images. With this approach, we were able to increase the performance of machine learning models and set a new benchmark amongst non-ensemble based models in skin lesion classification on the HAM10000 dataset; and used the observed analytics and generated models for detailed studies on model explainability, affirming the effectiveness of our solution.

Hyperspectral Demosaicing of Snapshot Camera Images Using Deep Learning

Spectral imaging technologies have rapidly evolved during the past decades. The recent development of single-camera-one-shot techniques for hyperspectral imaging allows multiple spectral bands to be captured simultaneously (3x3, 4x4 or 5x5 mosaic), opening up a wide range of applications. Examples include intraoperative imaging, agricultural field inspection and food quality assessment. To capture images across a wide spectrum range, i.e. to achieve high spectral resolution, the sensor design sacrifices spatial resolution. With increasing mosaic size, this effect becomes increasingly detrimental. Furthermore, demosaicing is challenging. Without incorporating edge, shape, and object information during interpolation, chromatic artifacts are likely to appear in the obtained images. Recent approaches use neural networks for demosaicing, enabling direct information extraction from image data. However, obtaining training data for these approaches poses a challenge as well. This work proposes a parallel neural network based demosaicing procedure trained on a new ground truth dataset captured in a controlled environment by a hyperspectral snapshot camera with a 4x4 mosaic pattern. The dataset is a combination of real captured scenes with images from publicly available data adapted to the 4x4 mosaic pattern. To obtain real world ground-truth data, we performed multiple camera captures with 1-pixel shifts in order to compose the entire data cube. Experiments show that the proposed network outperforms state-of-art networks.