Dual-Modality Computational Ophthalmic Imaging with Deep Learning and Coaxial Optical Design

The growing burden of myopia and retinal diseases necessitates more accessible and efficient eye screening solutions. This study presents a compact, dual-function optical device that integrates fundus photography and refractive error detection into a unified platform. The system features a coaxial optical design using dichroic mirrors to separate wavelength-dependent imaging paths, enabling simultaneous alignment of fundus and refraction modules. A Dense-U-Net-based algorithm with customized loss functions is employed for accurate pupil segmentation, facilitating automated alignment and focusing. Experimental evaluations demonstrate the system's capability to achieve high-precision pupil localization (EDE = 2.8 px, mIoU = 0.931) and reliable refractive estimation with a mean absolute error below 5%. Despite limitations due to commercial lens components, the proposed framework offers a promising solution for rapid, intelligent, and scalable ophthalmic screening, particularly suitable for community health settings.

Benchmarking the Cell Image Segmentation Models Robustness under the Microscope Optical Aberrations

Cell segmentation is essential in biomedical research for analyzing cellular morphology and behavior. Deep learning methods, particularly convolutional neural networks (CNNs), have revolutionized cell segmentation by extracting intricate features from images. However, the robustness of these methods under microscope optical aberrations remains a critical challenge. This study comprehensively evaluates the performance of cell instance segmentation models under simulated aberration conditions using the DynamicNuclearNet (DNN) and LIVECell datasets. Aberrations, including Astigmatism, Coma, Spherical, and Trefoil, were simulated using Zernike polynomial equations. Various segmentation models, such as Mask R-CNN with different network heads (FPN, C3) and backbones (ResNet, VGG19, SwinS), were trained and tested under aberrated conditions. Results indicate that FPN combined with SwinS demonstrates superior robustness in handling simple cell images affected by minor aberrations. Conversely, Cellpose2.0 proves effective for complex cell images under similar conditions. Our findings provide insights into selecting appropriate segmentation models based on cell morphology and aberration severity, enhancing the reliability of cell segmentation in biomedical applications. Further research is warranted to validate these methods with diverse aberration types and emerging segmentation models. Overall, this research aims to guide researchers in effectively utilizing cell segmentation models in the presence of minor optical aberrations.