What Does It Take to Detect an AI Agent? Minimal Feature Sets for Behavioral Detection under Browser Automation

Bot detectors deployed at scale treat traffic as binary: human or bot. This assumption breaks when AI agents browse the web through browser automation, a traffic class that is neither and that binary classifiers structurally cannot represent. We present a three-class detection framework distinguishing humans, bots, and AI agents, and show that the binary-vs-agent confusion is architectural: a binary human-vs-bot detector misroutes agent sessions because its label space lacks an agent class. On our controlled benchmark, an MLP binary classifier misclassifies 39.1% of real AI agents as human and a SAINT binary transformer misclassifies 34.5%; adding an explicit agent class yields per-class agent F1 = 1.000 in all 30 runs (3 model families $\times$ 10 seeds). To measure evasion resistance, we construct a five-level evasion ladder spanning passive observation, GAN-generated trajectories, and replay of real human cursor data ($n = 2299$ evasion sessions). Across 10 seeds and 3 model families we observe zero agent misses in 22990 per-seed predictions. The discriminative signal is a browser-automation artifact, not evidence of agent reasoning: Playwright does not emit the raw pointer-move and wheel-delta streams a physical input device produces, and this absence signature survives trajectory manipulation. Exhaustive search over all feature subsets of size 1-5 (9401 GBMs) shows that two behavioral features (mouse_event_rate, teleport_click_ratio) give 100% observed agent recall at every evasion level with agent precision 0.994; five features lift macro-F1 to 0.991. The signal is redundantly encoded: removing teleport_click_ratio leaves agent detection at 100%. The single-feature regime is degenerate, flagging every agent only by collapsing the classifier to always predict "agent". Two features robustly isolate agents; five separate all three traffic classes at macro-F1 $\geq 0.99$.

HASSL: Hierarchy-Aware Self-Supervised Learning Framework for Single Cell Microscopy

Hierarchical structure is common in image data, where fine-grained clusters often merge into larger, coarser semantic groups. In biological cell images, current self-supervised learning models often suppress this hierarchy, as coarse factors such as imaging modality can obscure finer morphological attributes in the latent space. We propose a hierarchy-aware self-supervised training framework to address this problem. Our method combines two components: a distillation framework with a segmentation teacher to improve morphological awareness in the latent space, and a hierarchy-aware contrastive loss based on HDBSCAN to improve decision boundaries between closely related subtypes at different hierarchical levels. Together, these components reduce the tendency of self-supervised learning to overemphasize coarse factors and instead align embeddings with semantic and morphological cues. This yields biologically meaningful sub-clusters driven by fine morphological detail. We train and evaluate our method on a curated corpus of 2.3 million single cells aggregated from 20 microscopy datasets, both labeled and unlabeled, covering 208 cell classes. Our method improves over baseline and counterpart methods, increasing average top-K accuracy by 2.8%, top-9 retrieval on the dataset with the deepest hierarchy by 6.3%, and downstream F1-score for biologically relevant drug classification from perturbed cell morphology by 7.8%.