SHREC 2021: Classification in cryo-electron tomograms

Cryo-electron tomography (cryo-ET) is an imaging technique that allows three-dimensional visualization of macro-molecular assemblies under near-native conditions. Cryo-ET comes with a number of challenges, mainly low signal-to-noise and inability to obtain images from all angles. Computational methods are key to analyze cryo-electron tomograms. To promote innovation in computational methods, we generate a novel simulated dataset to benchmark different methods of localization and classification of biological macromolecules in tomograms. Our publicly available dataset contains ten tomographic reconstructions of simulated cell-like volumes. Each volume contains twelve different types of complexes, varying in size, function and structure. In this paper, we have evaluated seven different methods of finding and classifying proteins. Seven research groups present results obtained with learning-based methods and trained on the simulated dataset, as well as a baseline template matching (TM), a traditional method widely used in cryo-ET research. We show that learning-based approaches can achieve notably better localization and classification performance than TM. We also experimentally confirm that there is a negative relationship between particle size and performance for all methods.

Deeply Cascaded U-Net for Multi-Task Image Processing

In current practice, many image processing tasks are done sequentially (e.g. denoising, dehazing, followed by semantic segmentation). In this paper, we propose a novel multi-task neural network architecture designed for combining sequential image processing tasks. We extend U-Net by additional decoding pathways for each individual task, and explore deep cascading of outputs and connectivity from one pathway to another. We demonstrate effectiveness of the proposed approach on denoising and semantic segmentation, as well as on progressive coarse-to-fine semantic segmentation, and achieve better performance than multiple individual or jointly-trained networks, with lower number of trainable parameters.