Automated detection of Zika and dengue in Aedes aegypti using neural spiking analysis

Mosquito-borne diseases present considerable risks to the health of both animals and humans. Aedes aegypti mosquitoes are the primary vectors for numerous medically important viruses such as dengue, Zika, yellow fever, and chikungunya. To characterize this mosquito neural activity, it is essential to classify the generated electrical spikes. However, no open-source neural spike classification method is currently available for mosquitoes. Our work presented in this paper provides an innovative artificial intelligence-based method to classify the neural spikes in uninfected, dengue-infected, and Zika-infected mosquitoes. Aiming for outstanding performance, the method employs a fusion of normalization, feature importance, and dimension reduction for the preprocessing and combines convolutional neural network and extra gradient boosting (XGBoost) for classification. The method uses the electrical spiking activity data of mosquito neurons recorded by microelectrode array technology. We used data from 0, 1, 2, 3, and 7 days post-infection, containing over 15 million samples, to analyze the method's performance. The performance of the proposed method was evaluated using accuracy, precision, recall, and the F1 scores. The results obtained from the method highlight its remarkable performance in differentiating infected vs uninfected mosquito samples, achieving an average of 98.1%. The performance was also compared with 6 other machine learning algorithms to further assess the method's capability. The method outperformed all other machine learning algorithms' performance. Overall, this research serves as an efficient method to classify the neural spikes of Aedes aegypti mosquitoes and can assist in unraveling the complex interactions between pathogens and mosquitoes.

Manipulating Soft Tissues by Deep Reinforcement Learning for Autonomous Robotic Surgery

In robotic surgery, pattern cutting through a deformable material is a challenging research field. The cutting procedure requires a robot to concurrently manipulate a scissor and a gripper to cut through a predefined contour trajectory on the deformable sheet. The gripper ensures the cutting accuracy by nailing a point on the sheet and continuously tensioning the pinch point to different directions while the scissor is in action. The goal is to find a pinch point and a corresponding tensioning policy to minimize damage to the material and increase cutting accuracy measured by the symmetric difference between the predefined contour and the cut contour. Previous study considers finding one fixed pinch point during the course of cutting, which is inaccurate and unsafe when the contour trajectory is complex. In this paper, we examine the soft tissue cutting task by using multiple pinch points, which imitates human operations while cutting. This approach, however, does not require the use of a multi-gripper robot. We use a deep reinforcement learning algorithm to find an optimal tensioning policy of a pinch point. Simulation results show that the multi-point approach outperforms the state-of-the-art method in soft pattern cutting task with respect to both accuracy and reliability.