HuiduRep: A Robust Self-Supervised Framework for Learning Neural Representations from Extracellular Spikes

Extracellular recordings are brief voltage fluctuations recorded near neurons, widely used in neuroscience as the basis for decoding brain activity at single-neuron resolution. Spike sorting, which assigns each spike to its source neuron, is a critical step in brain sensing pipelines. However, it remains challenging under low signal-to-noise ratio (SNR), electrode drift, and cross-session variability. In this paper, we propose HuiduRep, a robust self-supervised representation learning framework that extracts discriminative and generalizable features from extracellular spike waveforms. By combining contrastive learning with a denoising autoencoder, HuiduRep learns latent representations that are robust to noise and drift. Built on HuiduRep, we develop a spike sorting pipeline that clusters spike representations without supervision. Experiments on hybrid and real-world datasets demonstrate that HuiduRep achieves strong robustness and the pipeline matches or outperforms state-of-the-art tools such as KiloSort4 and MountainSort5. These findings demonstrate the potential of self-supervised spike representation learning as a foundational tool for robust and generalizable processing of extracellular recordings.