It remains challenging to achieve human-like locomotion in legged robots due to fundamental discrepancies between biological and mechanical structures. Although imitation learning has emerged as a promising approach for generating natural robotic movements, simply replicating joint angle trajectories fails to capture the underlying principles of human motion. This study proposes a Gait Divergence Analysis Framework (GDAF), a unified biomechanical evaluation framework that systematically quantifies kinematic and kinetic discrepancies between humans and bipedal robots. We apply GDAF to systematically compare human and humanoid locomotion across 28 walking speeds. To enable reproducible analysis, we collect and release a speed-continuous humanoid locomotion dataset from a state-of-the-art humanoid controller. We further provide an open-source implementation of GDAF, including analysis, visualization, and MuJoCo-based tools, enabling quantitative, interpretable, and reproducible biomechanical analysis of humanoid locomotion. Results demonstrate that despite visually human-like motion generated by modern humanoid controllers, significant biomechanical divergence persists across speeds. Robots exhibit systematic deviations in gait symmetry, energy distribution, and joint coordination, indicating that substantial room remains for improving the biomechanical fidelity and energetic efficiency of humanoid locomotion. This work provides a quantitative benchmark for evaluating humanoid locomotion and offers data and versatile tools to support the development of more human-like and energetically efficient locomotion controllers. The data and code will be made publicly available upon acceptance of the paper.