At FullTilt: Real-Time Open-Set 3D Macromolecule Detection Directly from Tilted 2D Projections

Open-set 3D macromolecule detection in cryogenic electron tomography eliminates the need for target-specific model retraining. However, strict VRAM constraints prohibit processing an entire 3D tomogram, forcing current methods to rely on slow sliding-window inference over extracted subvolumes. To overcome this, we propose FullTilt, an end-to-end framework that redefines 3D detection by operating directly on aligned 2D tilt-series. Because a tilt-series contains significantly fewer images than slices in a reconstructed tomogram, FullTilt eliminates redundant volumetric computation, accelerating inference by orders of magnitude. To process the entire tilt-series simultaneously, we introduce a tilt-series encoder to efficiently fuse cross-view information. We further propose a multiclass visual prompt encoder for flexible prompting, a tilt-aware query initializer to effectively anchor 3D queries, and an auxiliary geometric primitives module to enhance the model's understanding of multi-view geometry while improving robustness to adverse imaging artifacts. Extensive evaluations on three real-world datasets demonstrate that FullTilt achieves state-of-the-art zero-shot performance while drastically reducing runtime and VRAM requirements, paving the way for rapid, large-scale visual proteomics analysis. All code and data will be publicly available upon publication.

Every Pixel Has its Moments: Ultra-High-Resolution Unpaired Image-to-Image Translation via Dense Normalization

Recent advancements in ultra-high-resolution unpaired image-to-image translation have aimed to mitigate the constraints imposed by limited GPU memory through patch-wise inference. Nonetheless, existing methods often compromise between the reduction of noticeable tiling artifacts and the preservation of color and hue contrast, attributed to the reliance on global image- or patch-level statistics in the instance normalization layers. In this study, we introduce a Dense Normalization (DN) layer designed to estimate pixel-level statistical moments. This approach effectively diminishes tiling artifacts while concurrently preserving local color and hue contrasts. To address the computational demands of pixel-level estimation, we further propose an efficient interpolation algorithm. Moreover, we invent a parallelism strategy that enables the DN layer to operate in a single pass. Through extensive experiments, we demonstrate that our method surpasses all existing approaches in performance. Notably, our DN layer is hyperparameter-free and can be seamlessly integrated into most unpaired image-to-image translation frameworks without necessitating retraining. Overall, our work paves the way for future exploration in handling images of arbitrary resolutions within the realm of unpaired image-to-image translation. Code is available at: https://github.com/Kaminyou/Dense-Normalization.

Ultra-high-resolution unpaired stain transformation via Kernelized Instance Normalization

While hematoxylin and eosin (H&E) is a standard staining procedure, immunohistochemistry (IHC) staining further serves as a diagnostic and prognostic method. However, acquiring special staining results requires substantial costs. Hence, we proposed a strategy for ultra-high-resolution unpaired image-to-image translation: Kernelized Instance Normalization (KIN), which preserves local information and successfully achieves seamless stain transformation with constant GPU memory usage. Given a patch, corresponding position, and a kernel, KIN computes local statistics using convolution operation. In addition, KIN can be easily plugged into most currently developed frameworks without re-training. We demonstrate that KIN achieves state-of-the-art stain transformation by replacing instance normalization (IN) layers with KIN layers in three popular frameworks and testing on two histopathological datasets. Furthermore, we manifest the generalizability of KIN with high-resolution natural images. Finally, human evaluation and several objective metrics are used to compare the performance of different approaches. Overall, this is the first successful study for the ultra-high-resolution unpaired image-to-image translation with constant space complexity. Code is available at: https://github.com/Kaminyou/URUST