ProfOlaf: Semi-Automated Tool for Systematic Literature Reviews

Systematic reviews and mapping studies are critical for synthesizing research, identifying gaps, and guiding future work, but they are often labor-intensive and time-consuming. Existing tools provide partial support for specific steps, leaving much of the process manual and error-prone. We present ProfOlaf, a semi-automated tool designed to streamline systematic reviews while maintaining methodological rigor. ProfOlaf supports iterative snowballing for article collection with human-in-the-loop filtering and uses large language models to assist in analyzing articles, extracting key topics, and answering queries about the content of papers. By combining automation with guided manual effort, ProfOlaf enhances the efficiency, quality, and reproducibility of systematic reviews across research fields. A video describing and demonstrating ProfOlaf is available at: https://youtu.be/4noUXfcmxsE

Finding Regions of Interest in Whole Slide Images Using Multiple Instance Learning

Whole Slide Images (WSI), obtained by high-resolution digital scanning of microscope slides at multiple scales, are the cornerstone of modern Digital Pathology. However, they represent a particular challenge to AI-based/AI-mediated analysis because pathology labeling is typically done at slide-level, instead of tile-level. It is not just that medical diagnostics is recorded at the specimen level, the detection of oncogene mutation is also experimentally obtained, and recorded by initiatives like The Cancer Genome Atlas (TCGA), at the slide level. This configures a dual challenge: a) accurately predicting the overall cancer phenotype and b) finding out what cellular morphologies are associated with it at the tile level. To address these challenges, a weakly supervised Multiple Instance Learning (MIL) approach was explored for two prevalent cancer types, Invasive Breast Carcinoma (TCGA-BRCA) and Lung Squamous Cell Carcinoma (TCGA-LUSC). This approach was explored for tumor detection at low magnification levels and TP53 mutations at various levels. Our results show that a novel additive implementation of MIL matched the performance of reference implementation (AUC 0.96), and was only slightly outperformed by Attention MIL (AUC 0.97). More interestingly from the perspective of the molecular pathologist, these different AI architectures identify distinct sensitivities to morphological features (through the detection of Regions of Interest, RoI) at different amplification levels. Tellingly, TP53 mutation was most sensitive to features at the higher applications where cellular morphology is resolved.