Analyzing Examinee Comments using DistilBERT and Machine Learning to Ensure Quality Control in Exam Content

This study explores using Natural Language Processing (NLP) to analyze candidate comments for identifying problematic test items. We developed and validated machine learning models that automatically identify relevant negative feedback, evaluated approaches of incorporating psychometric features enhances model performance, and compared NLP-flagged items with traditionally flagged items. Results demonstrate that candidate feedback provides valuable complementary information to statistical methods, potentially improving test validity while reducing manual review burden. This research offers testing organizations an efficient mechanism to incorporate direct candidate experience into quality assurance processes.

Beyond Current Boundaries: Integrating Deep Learning and AlphaFold for Enhanced Protein Structure Prediction from Low-Resolution Cryo-EM Maps

Constructing atomic models from cryo-electron microscopy (cryo-EM) maps is a crucial yet intricate task in structural biology. While advancements in deep learning, such as convolutional neural networks (CNNs) and graph neural networks (GNNs), have spurred the development of sophisticated map-to-model tools like DeepTracer and ModelAngelo, their efficacy notably diminishes with low-resolution maps beyond 4 {\AA}. To address this shortfall, our research introduces DeepTracer-LowResEnhance, an innovative framework that synergizes a deep learning-enhanced map refinement technique with the power of AlphaFold. This methodology is designed to markedly improve the construction of models from low-resolution cryo-EM maps. DeepTracer-LowResEnhance was rigorously tested on a set of 37 protein cryo-EM maps, with resolutions ranging between 2.5 to 8.4 {\AA}, including 22 maps with resolutions lower than 4 {\AA}. The outcomes were compelling, demonstrating that 95.5\% of the low-resolution maps exhibited a significant uptick in the count of total predicted residues. This denotes a pronounced improvement in atomic model building for low-resolution maps. Additionally, a comparative analysis alongside Phenix's auto-sharpening functionality delineates DeepTracer-LowResEnhance's superior capability in rendering more detailed and precise atomic models, thereby pushing the boundaries of current computational structural biology methodologies.