Inexpensive Optical Projection Tomography on a Mobile Phone Platform

This work presents an inexpensive optical projection tomography (OPT) system built on a mobile phone platform for three-dimensional optical microscopy. The system uses an iPhone camera together with a low-cost commercial microscope lens attachment, a stepper motor for sample rotation, LED illumination, and custom 3D-printed components, with a total component cost of approximately 50 US dollars excluding the phone. To support system evaluation, we also developed a low-cost method for fabricating a zebrafish phantom by embedding fixed larvae in UV-cured resin. Camera calibration was performed using a checkerboard target, and effective magnification was estimated with images of a 1951 Air Force resolution target. Projection images acquired during sample rotation were converted to attenuation images and corrected for field nonuniformity. Each slice was reconstructed with filtered backprojection and the resulting slices were stacked into a 3D volume. The completed system achieved a resolution of 3.91 $μm$ and produced volumetric reconstructions in which anatomical features of the zebrafish phantom, including the spine, were clearly visible. These results demonstrate that mobile-phone-based OPT can provide accessible, portable, and low-cost 3D microscopy, with potential utility for education, field work, and resource-limited settings.

SPIRiT Regularization: Parallel MRI with a Combination of Sensitivity Encoding and Linear Predictability

Accelerated Magnetic Resonance Imaging (MRI) permits high quality images from fewer samples that can be collected with a faster scan. Two established methods for accelerating MRI include parallel imaging and compressed sensing. Two types of parallel imaging include linear predictability, which assumes that the Fourier samples are linearly related, and sensitivity encoding, which incorporates a priori knowledge of the sensitivity maps. In this work, we combine compressed sensing with both types of parallel imaging using a novel regularization term: SPIRiT regularization. When combined, the reconstructed images are improved. We demonstrate results on data of a brain, a knee, and an ankle.