In this paper, we have carried out the detail studies of pre-cancer by wavelet coherency and multifractal based detrended fluctuation analysis (MFDFA) on differential interference contrast (DIC) images of stromal region among different grades of pre-cancer tissues. Discrete wavelet transform (DWT) through Daubechies basis has been performed for identifying fluctuations over polynomial trends for clear characterization and differentiation of tissues. Wavelet coherence plots are performed for identifying the level of correlation in time scale plane between normal and various grades of DIC samples. Applying MFDFA on refractive index variations of cervical tissues, we have observed that the values of Hurst exponent (correlation) decreases from healthy (normal) to pre-cancer tissues. The width of singularity spectrum has a sudden degradation at grade-I in comparison of healthy (normal) tissue but later on it increases as cancer progresses from grade-II to grade-III.
CT scan images of human brain of a particular patient in different cross sections are taken, on which wavelet transform and multi-fractal analysis are applied. The vertical and horizontal unfolding of images are done before analyzing these images. A systematic investigation of de-noised CT scan images of human brain in different cross-sections are carried out through wavelet normalized energy and wavelet semi-log plots, which clearly points out the mismatch between results of vertical and horizontal unfolding. The mismatch of results confirms the heterogeneity in spatial domain. Using the multi-fractal de-trended fluctuation analysis (MFDFA), the mismatch between the values of Hurst exponent and width of singularity spectrum by vertical and horizontal unfolding confirms the same.