Detection of sub-cellular changes by use of $λ=6.5$ micron laser light interaction in association with Intelligent Laser Speckle Classification technique

The study is based on a principle of laser physics so that a (coherent) laser light whose wavelength is shorter than a feature under inspection (like sub-cellular component) can interact with such specific feature (or textural features) and generates laser speckle patterns which can characterize those specific features. By the method we have managed to detect differences at sub-cellular scales such as genetic modification, cellular shape deformation, etc. with 87% accuracy. In this study red laser is used whose wavelength (6.5 microns) is shorter than a plant cell (~60 microns) that is suitable to interact with sub-cellular features. The work is assumed to be an initial stage of further application on human cellular changes observation that would be utilized for development of more accurate methods such as better drug delivery assessments, systemic diseases early diagnosis, etc.